FISSION YEAST GENES INVOLVED IN COUPLING MITOSIS TO COMPLETION OF DNA-REPLICATION
FISSION YEAST GENES INVOLVED IN COUPLING MITOSIS TO COMPLETION OF DNA-REPLICATION
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DOI：
10.1101/gad.6.11.2035
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发表时间：
1992-11-01
影响因子：
10.5
通讯作者：
NURSE, P
中科院分区：
文献类型：
--
作者：
ENOCH, T;CARR, AM;NURSE, P
我们已经分离出分裂酵母突变体，当DNA复制被羟基脲阻断时，它们进入有丝分裂。突变体定义了8个连锁群，其中3个由rad 1、rad 3和rad 17基因的等位基因组成。最近，这些裂变酵母基因已被证明是辐射诱导的细胞周期停滞所需的，正如芽殖酵母RAD 9基因一样。其他五个基因被称为hus（hydroxyurea sensitive）1-5。我们建议，这些基因参与细胞内的信号转导途径，监测完成DNA复制和有丝分裂控制蛋白cdc 2的信息传输。绕过cdc 25（有丝分裂调节因子cdc 2的激活因子）的突变也会使有丝分裂与DNA复制分离。然而，在cdc 25基因缺失的菌株中，有丝分裂会被DNA复制抑制剂阻断，这表明尽管cdc 25影响有丝分裂与DNA复制完成的偶联，但它对于这种控制并不是必需的。
We have isolated fission yeast mutants that enter mitosis when DNA replication is blocked with hydroxyurea. The mutants define eight linkage groups, three of which consist of alleles of the rad1, rad3, and rad17 genes. Recently, these fission yeast genes have been shown to be required for radiation-induced cell cycle arrest, as is the budding yeast RAD9 gene. The other five genes are called hus (hydroxyurea sensitive) 1-5. We propose that these genes participate in an intracellular signal transduction pathway that monitors the completion of DNA replication and transmits information to the mitotic control protein cdc2. Mutations that bypass the requirement for cdc25 (an activator of the mitotic regulator cdc2) also uncouple mitosis from DNA replication. However, mitosis is blocked by inhibitors of DNA replication in strains in which the cdc25 gene has been deleted, indicating that although cdc25 influences the coupling of mitosis to the completion of DNA replication, it is not essential for this control.