Pig cloning by microinjection of fetal fibroblast nuclei
Pig cloning by microinjection of fetal fibroblast nuclei
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DOI：
10.1126/science.289.5482.1188
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发表时间：
2000-08-18
期刊：
影响因子：
56.9
通讯作者：
Perry, ACF
中科院分区：
文献类型：
--
作者：
Onishi, A;Iwamoto, M;Perry, ACF
猪的克隆将对肉类生产的优化和异种移植产生显著的影响。为了从分化的细胞中克隆猪，我们将猪(Sus Scrofa)胎儿成纤维细胞的细胞核显微注射到去核的卵母细胞中，并通过电激活诱导发育。将110个克隆胚胎转移给四个代孕母亲，产生了一只看起来正常的雌性小猪。该仔猪的克隆种源由其毛色指示，并经DNA微卫星分析证实。
Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.