Identification of chitinase genes and roles in the larval-pupal transition of Leptinotarsa decemlineata
Identification of chitinase genes and roles in the larval-pupal transition of Leptinotarsa decemlineata
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DOI：
10.1002/ps.7754
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发表时间：
2023-09-20
影响因子：
4.1
通讯作者：
Shi，Ji-Feng
中科院分区：
文献类型：
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作者：
Chen，Yu;Tang，Hong;Shi，Ji-Feng
昆虫几丁质酶在降解细胞外基质中的几丁质，影响昆虫的发育和蜕皮中起着重要作用。然而，我们对马铃薯叶甲中各种几丁质酶的特定功能的了解有限，阻碍了新的基因打靶技术作为害虫管理策略的部署。尽管有不同的结构域架构，所有这些几丁质酶含有至少一个几丁质酶催化结构域。系统发育分析将几丁质酶蛋白分为10个不同的簇（组I-X）。表达谱显示在富含几丁质的组织中或在从幼虫到蛹过渡的特定发育阶段中表达最高。基因特异性RNA干扰（RNAi）实验为几丁质酶基因功能提供了有价值的见解。沉默组IILDCht 10阻止幼虫蜕皮，幼虫prepupal，和prepupal-pupal过程。此外，我们的研究表明，LdCht 5，LdCht 2，LdCht 11，LdCht 1和LdCht 3从I和VII-X组是特别必要的预蛹到蛹的过渡阶段，而LdIDGF 2从V组是必要的幼虫-预蛹变态过程。第三组的几丁质酶基因LdCht 7和第五组的LdIDGF 4参与了幼虫到预蛹和预蛹到蛹的转移。此外，我们的研究结果还揭示了第四组内的9个几丁质酶基因在消化系统中的独家表达，表明它们在调节幼虫体重和幼虫到蛹transition. CONCLUSION的潜在作用我们的研究结果提供了一个全面的了解几丁质酶基因的功能专业化在蜕皮过程中的各个阶段，并确定潜在的目标为基础的RNAi管理ofLeptinotarsa decemlineata。© 2023化学工业协会。
BACKGROUNDInsect chitinases play crucial roles in degrading chitin in the extracellular matrix, affecting insect development and molting. However, our understanding of the specific functions of various chitinases inLeptinotarsa decemlineatais limited, hindering the deployment of novel gene‐targeting technologies as pest management strategies.RESULTSWe identified and characterized 19 full‐length complementary DNA (cDNA) sequences of chitinase genes (LdChts) inLeptinotarsa decemlineata. Despite having varying domain architectures, all these chitinases contained at least one chitinase catalytic domain. Phylogenetic analysis classified the chitinase proteins into ten distinct clusters (groups I–X). Expression profiles showed the highest expression in chitin‐rich tissues or during specific developmental stages from the larva‐to‐pupa transition. Gene‐specific RNA interference (RNAi) experiments provided valuable insight into chitinase gene function. Silencing of group IILdCht10prevented larval–larval molting, larval–prepupal, and prepupal–pupal processes. Moreover, our study revealed thatLdCht5,LdCht2,LdCht11,LdCht1, andLdCht3from groups I and VII–X were specifically essential for the transition from prepupal to pupal stage, whereasLdIDGF2from group V was necessary for the larval–prepupal metamorphic process. The chitinase geneLdCht7from group III andLdIDGF4from group V were involved in both the larva‐to‐prepupa and the prepupa‐to‐pupa shift. Additionally, our findings also shed light on the exclusive expression of nine chitinase genes within group IV in the digestive system, suggesting their potential role in regulating larval body weight and larva‐to‐pupa transition.CONCLUSIONOur results provide a comprehensive understanding of the functional specialization of chitinase genes during the molting process of various stages and identify potential targets for RNAi‐based management ofLeptinotarsa decemlineata. © 2023 Society of Chemical Industry.