Yeast cells proliferation on various strong static magnetic fields and temperatures
Yeast cells proliferation on various strong static magnetic fields and temperatures
复制标题
酵母细胞在各种强静磁场和温度下增殖
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
H. Hayashi
中科院分区：
文献类型：
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作者：
E S Otabe;S. Kuroki;J. Nikawa;Y. Matsumoto;T. Ooba;K. Kiso;H. Hayashi
研究了强磁场对酵母细胞活力的影响。实验用酵母细胞在5 ml YPD（酵母提取物蛋白胨葡萄糖）中培养，酵母细胞数密度为5.0 ±0.2 × 106/ml，温度和磁场最高可达10 T。由于酵母细胞被放置在超导磁体的中心，由于抗磁性和磁梯度引起的磁力的影响小得可以忽略不计。将酵母悬浮液暴露于空气中并在振荡条件下培养。用带有光学显微镜的计数板对酵母悬浮液中的酵母细胞数进行计数，并测量酵母细胞数密度的时间依赖性。根据马尔萨斯方程ρ = ρ oexp（kt），分析了初始部分酵母细胞数密度ρ随时间的变化关系，其中k为生长系数。结果表明，与对照相比，磁场下的生长系数受到抑制。生长系数随磁场强度的增加而减小，在5 T左右达到饱和.另一方面，发现磁场对酵母细胞生长的抑制在高温下减弱。
The effect of strong magnetic fields on activities of yeast cells were investigated. Experimental yeast cells were cultured in 5 ml of YPD(Yeast extract Peptone Dextrose) for the number density of yeast cells of 5.0 ±0.2 x 106/ml with various temperatures and magnetic fields up to 10 T. Since the yeast cells were placed in the center of the superconducting magnet, the effect of magnetic force due to the diamagnetism and magnetic gradient was negligibly small. The yeast suspension was opened to air and cultured in shaking condition. The number of yeast cells in the yeast suspension was counted by a counting plate with an optical microscope, and the time dependence of the number density of yeast cells was measured. The time dependence of the number density of yeast cells, ρ, of initial part is analyzed in terms of Malthus equation as given by ρ = ρo exp(kt), where k is the growth coefficient. It is found that, the growth coefficient under the magnetic field is suppressed compared with the control. The growth coefficient decreasing as increasing magnetic field and is saturated at about 5 T. On the other hand, it is found that the suppression of growth of yeast cells by the magnetic field is diminished at high temperatures.