Effect of Therapeutic Ultrasound on the Release of Insulin, Glucagon, and Alpha-Amylase from Ex Vivo Pancreatic Models.
Effect of Therapeutic Ultrasound on the Release of Insulin, Glucagon, and Alpha-Amylase from Ex Vivo Pancreatic Models.
复制标题
治疗性超声对离体胰腺模型释放胰岛素、胰高血糖素和 α-淀粉酶的影响。
DOI：
10.1002/jum.15661
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Zderic,Vesna
中科院分区：
文献类型：
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作者：
Suarez-Castellanos,Ivan;Singh,Tania;ChatterjeeBhowmick,Diti;Cohen,Joshua;Jeremic,Aleksandar;Zderic,Vesna
我们之前发表的研究表明，治疗性超声（US）作为治疗2型糖尿病分泌不足的一种新的非药物替代方法具有潜力。尽管显示β细胞胰岛素释放增强，但这些研究并未探索US治疗对朗格汉斯胰岛其他细胞的潜在影响，如分泌胰高血糖素的α细胞或外分泌胰腺的腺泡细胞。方法采用能安全刺激胰腺β细胞分泌胰岛素的US参数（f= 800 kHz，ISPTA= 0.5-1 W/cm2, 5分钟），建立兔胰腺切丁模型（每组6只）。通过在治疗开始前（t= 0分钟）、治疗后立即（t= 5分钟）和治疗结束后30分钟（t= 35分钟）收集细胞外培养基的等分来测量US治疗后胰岛素和胰高血糖素的释放量。在兔胰腺实验中评估了US处理后消化酶α -淀粉酶的潜在释放。在少量人胰岛培养皿中也进行了初步研究（每组n= 3）。通过组织学分析评估US处理过的兔胰腺组织和人胰岛的总体完整性。结果经假处理的兔胰腺样品细胞外胰岛素含量降低，但在800 kHz和1 W/cm2的US作用下，5分钟后胰岛素释放量增加（P< 0.005）。此外，35分钟后未检测到胰岛素进一步释放。细胞外胰高血糖素和α -淀粉酶浓度在US治疗组和假兔胰腺组之间无统计学差异。对人类胰岛的初步研究似乎遵循了在兔胰腺研究中观察到的趋势。胰岛和其他胰腺组织的完整性似乎没有受到美国治疗的影响。结论一种潜在的基于美国的促进胰岛素释放的策略需要优化胰腺β细胞的胰岛素分泌，同时减少胰高血糖素和胰酶的分泌。
ObjectivesOur previously published studies showed the potential of therapeutic ultrasound (US) as a novel non‐pharmacological alternative for the treatment of secretory deficiencies in type 2 diabetes. Despite showing enhanced insulin release from beta cells, these studies did not explore the potential effects of US treatment on other cells in the islets of Langerhans such as glucagon‐secreting alpha cells or acinar cells of the exocrine pancreas.MethodsWe applied US parameters found capable of safely stimulating insulin secretion from pancreatic beta cells (f= 800 kHz,ISPTA= 0.5–1 W/cm2, 5 minutes) to a diced rabbit pancreas model in culture plates (n= 6 per group). Released quantities of insulin and glucagon in response to US treatment were measured by collecting aliquots of the extracellular medium prior to the start of the treatment (t= 0 minute), immediately after treatment (t= 5 minutes) and 30 minutes after the end of treatment (t= 35 minutes). Potential release of digestive enzyme alpha‐amylase as a result of US treatment was evaluated in rabbit pancreas experiments. Preliminary studies were also performed in a small number of human pancreatic islets in culture plates (n= 3 per group). The general integrity of the US‐treated rabbit pancreatic tissue and human pancreatic islets was evaluated through histological analysis.ResultsWhile sham‐treated rabbit pancreas samples showed decreased extracellular insulin content, there was an increase in insulin release att= 5 minutes from samples treated with US at 800 kHz and 1 W/cm2(P<.005). Furthermore, no further insulin release was detected att= 35 minutes. No statistically significant difference in extracellular glucagon and alpha‐amylase concentrations was observed between US‐treated and sham rabbit pancreas groups. Preliminary studies in human islets appeared to follow trends observed in rabbit pancreas studies. Islet and other pancreatic tissue integrity did not appear to be affected by the US treatment.ConclusionA potential US‐based strategy for enhanced insulin release would require optimization of insulin secretion from pancreatic beta cells while minimizing glucagon and pancreatic enzyme secretions.