Use of domain-deletion mutants to locate Lutheran blood group antigens to each of the five immunoglobulin superfamily domains of the Lutheran glycoprotein: Elucidation of the molecular basis of the Lu-a/Lu-b and the Au-a/Au-b polymorphisms
Use of domain-deletion mutants to locate Lutheran blood group antigens to each of the five immunoglobulin superfamily domains of the Lutheran glycoprotein: Elucidation of the molecular basis of the Lu-a/Lu-b and the Au-a/Au-b polymorphisms
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DOI：
10.1182/blood.v89.11.4219
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发表时间：
1997-06-01
期刊：
影响因子：
20.3
通讯作者：
Anstee, DJ
中科院分区：
文献类型：
--
作者：
Parsons, SF;Mallinson, G;Anstee, DJ
路德糖蛋白 (Lu gp) 有五个预测的免疫球蛋白超家族 (IgSF) 结构域。将Lu cDNA转染K562细胞，并用单克隆抗体和Lu血型抗血清进行流式细胞术检测，结果证实了Lu cDNA的身份。通过反向聚合酶链式反应 (PCR) 制备缺乏编码一个或多个 IgSF 结构域的区域的缺失突变体，在 K562 细胞中表达，并用相同的抗体进行测试。 Lu-b 和 Lu5 抗原以及单克隆抗体 BRIC 224 识别的表位被定位到第一个 N 端 IgSF 结构域。 Lu4和Lu8映射到域2； Lu20至域3； Lu7 和 BRIC 221 表位位于结构域 4，Lu13 和 Au-b 位于结构域 5。确定了 LU 基因的组织。编码开放阅读框的区域排列在染色体 19q13.2 上延伸约 11 kb 的 15 个外显子中。使用分型献血者的基因组 DNA 研究了 Lu-a/Lu-b 和 Au-a/Au-b 血型多态性。 Lu-a 突变是外显子 3 中的碱基变化（G(252) 至 A），编码结构域 1 的 CFG 面上的 Arg(77) (Lu-b) 至 His (Lu-a) 变化。Au-a/Au-b 多态性是外显子 12 中的 A(1637) 至 G 替换，编码结构域 G 链上的 Thr(539) (Au-a) 至 Ala (Au-b) 变化5. (C) 1997 年，美国血液学会。
Lutheran glycoprotein (Lu gp) has five predicted immunoglobulin superfamily (IgSF) domains. K562 cells were transfected with Lu cDNA and tested by flow cytometry with monoclonal antibodies and Lu blood group antisera, The results confirmed the identity of Lu cDNA. Deletion mutants lacking the regions encoding one or more IgSF domains were made by inverse polymerase chain reaction (PCR), expressed in K562 cells, and tested with the same antibodies. The Lu-b and Lu5 antigens and the epitope recognized by monoclonal antibody BRIC 224 were mapped to the first, N-terminal, IgSF domain. Lu4 and Lu8 were mapped to domain 2; Lu20 to domain 3; Lu7 and BRIC 221 epitope to domain 4, and Lu13 and Au-b to domain 5. The organization of the LU gene was determined. The region encoding the open reading frame is arranged in 15 exons extending over approximate to 11 kb on chromosome 19q13.2. The Lu-a/Lu-b and Au-a/Au-b blood group polymorphisms were studied using genomic DNA from typed blood donors. The Lu-a mutation is a base change in exon 3 (G(252) to A) encoding an Arg(77) (Lu-b) to His (Lu-a) change on the CFG face of domain 1. The Au-a/Au-b polymorphism is an A(1637) to G substitution in exon 12 encoding a Thr(539) (Au-a) to Ala (Au-b) change on the G strand of domain 5. (C) 1997 by The American Society of Hematology.