Hydrogenophilus thiooxidans sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats
Hydrogenophilus thiooxidans sp. nov., a moderately thermophilic chemotrophic bacterium unable to grow on hydrogen gas, isolated from hot spring microbial mats
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嗜氢氧化硫杆菌 sp.
DOI：
10.1099/ijsem.0.005355
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发表时间：
2022
影响因子：
2.8
通讯作者：
Haruta Shin
中科院分区：
文献类型：
--
作者：
Kawai Shigeru;Ishikawa Mikako;Hanada Satoshi;Haruta Shin
从日本中房温泉中分离到一株新的嗜热化能营养菌SS 56 T。分离株为杆状（1.5-2.1×0.6-0.8 µm）革兰氏染色阴性细菌。该菌株的细胞在好氧和厌氧条件下生长。在有氧条件下，硫代硫酸盐和元素硫，但不与H2作为电子供体的自养生长观察。在O2存在下，酵母膏、胰蛋白胨、聚蛋白胨和有机酸均能产生异养生长。菌株SS 56 T在厌氧化学异养条件下利用亚硝酸盐作为电子受体。该菌株在35 ~ 65 °C之间生长，最适温度为55 °C。生长的pH范围为pH 6.0-9.0;最佳生长发生在pH 7.0-8.0。菌株SS 56 T的16 S rRNA基因序列与嗜热嗜氢菌TH-1 T的16 S rRNA基因序列同源性为98.9%。SS 56 T株基因组草图序列为2401804 bp，与H株基因组序列2223143 bp相比，其DNA-DNA数字杂交率为53.7%，核苷酸同源性为92.9%，氨基酸同源性为93.6%。热释光TH-1 T。基于上述信息，提出菌株SS 56 T（=DSM 111892 T =JCM 34254 T）作为新物种氧化硫嗜氢菌的模式菌株。Nov.
A novel thermophilic chemotrophic bacterium, strain SS56T, was isolated from Nakabusa Hot Spring, Japan. The isolate was a rod-shaped (1.5–2.1×0.6–0.8 µm), Gram-stain-negative bacterium. The cells of this strain grew chemoheterotrophically under aerobic and anaerobic conditions. Autotrophic growth was observed with thiosulphate and elemental sulphur under aerobic conditions but not with H2as the electron donor. Heterotrophic growth in the presence of O2occurred on yeast extract, tryptone, polypeptone and organic acids. Strain SS56Tused nitrite as an alternative electron acceptor under anaerobic chemoheterotrophic conditions. The isolate grew between 35 and 65 °C, with the optimum at 55 °C. The pH range for growth was pH 6.0–9.0; optimal growth occurred at pH 7.0–8.0. The 16S rRNA gene sequence of strain SS56Twas 98.9% identical to that ofHydrogenophilus thermoluteolusTH-1T. The draft genome sequence of 2401804 bp for strain SS56Tgave values of 53.7% for digital DNA–DNA hybridization, 92.9% for average nucleotide identity and 93.6% for average amino acid identity compared with the genome sequence of 2223143 bp forH. thermoluteolusTH-1T. Based on the information described above, strain SS56T(=DSM 111892T=JCM 34254T) is proposed as the type strain of a novel species,Hydrogenophilus thiooxidanssp. nov.