RAPID METHOD FOR DETERMINATION OF TOTAL FATTY-ACID CONTENT AND COMPOSITION OF FEEDSTUFFS AND FECES
RAPID METHOD FOR DETERMINATION OF TOTAL FATTY-ACID CONTENT AND COMPOSITION OF FEEDSTUFFS AND FECES
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DOI：
10.1021/jf00084a019
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发表时间：
1988-11-01
影响因子：
6.1
通讯作者：
PALMQUIST, DL
中科院分区：
文献类型：
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作者：
SUKHIJA, PS;PALMQUIST, DL
目前用于定量饲料中总脂肪和脂肪酸组成的方法需要溶剂萃取、纯化和酯化，然后进行气相色谱分析。本文介绍了一种用5.0mL甲醇-苯（或氯仿）-乙酰氯（20：27：3）组成的混合溶剂，对50-500 mg含10-50 mg脂肪酸的样品进行2小时一步萃取-酯交换的方法，该方法对许多饲料、油料、钙皂、乳脂和粪便样品的脂肪酸含量和组成给出了可重复的结果。该程序也是有用的定量脂肪酸提取的油索氏和脂质类分离的薄层色谱法。一步提取-酯交换法简单、快速、方便、定量;脂肪酸被具体测定，提供脂肪营养价值的精确估计。该方法对于研究和商业目的都是有用的。
Methods currently used for quantitating total fat and fatty acid composition in feedstuffs require solvent extraction, purification,and esterification followed by gas chromatographic analysis. A 2-h, one-step extraction-transesterification procedure using 5.0 mL of solvent mixture consisting of methanol-benzene (or chloroform)-acetyl chloride (20:27:3) for 50-500 mg of sample containing 10-50 mg of fatty acids is described, which gave reproducible results for fatty acid content and composition of many feedstuffs, oilseeds, calcium soap, milk fat, and feces samples. The procedure also was useful for quantitating fatty acids of oil extracted by Soxhlet and of lipid classes separated by thin-layer chromatography. The one-step extraction- transesterification procedure is simple, rapid, convenient, and quantitative; fatty acids are determined specifically, providing a precise estimate of nutritative value of fats. The method is useful both for research and commercial purposes.