Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells.
Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells.
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在同步 CV-1 细胞中通过高 Mr 载体 DNA 增强 DNA 介导的基因转移。
DOI：
10.1042/bj2250529
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
A. Wyllie
中科院分区：
文献类型：
--
作者：
A. Strain;W. A. Wallace;A. Wyllie
用SV 40（猿猴病毒40）DNA-磷酸钙共沉淀物转染同步化CV-1细胞。在载体DNA的存在下，SV 40 DNA的转染效率降低5倍，在S期细胞和增加4倍，有丝分裂富集细胞的制剂相比，异步控制。当载体DNA被省略时，当细胞已经通过S期进入G2期时，或者当检测细胞对完整的SV 40病毒的感染性时，没有观察到差异。这些结果突出了细胞周期依赖因子对DNA介导的基因转移的重要性。
Synchronized CV-1 cells were transfected with SV40 (simian virus 40) DNA-calcium phosphate co-precipitates. In the presence of carrier DNA, the transfection efficiency of SV40 DNA was decreased 5-fold in S-phase cells and was increased 4-fold in preparations of mitotically enriched cells as compared with asynchronous controls. No difference was observed when carrier DNA was omitted, when cells had progressed through S-phase and into G2-phase, or when the infectivity of cells to intact SV40 virus was tested. These results highlight the importance of cell-cycle-dependent factors on DNA-mediated gene transfer.