The HIV-1 Nef protein interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA.
The HIV-1 Nef protein interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA.
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DOI：
10.1186/1743-422x-9-103
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发表时间：
2012-07-10
期刊：
影响因子：
4.8
通讯作者：
Herbein G
中科院分区：
文献类型：
--
作者：
Abbas W;Dichamp I;Herbein G
人类免疫缺陷病毒1型（HIV-1）Nef编码的蛋白在病毒生命周期的几乎所有阶段都起着关键作用，但其在翻译中的作用在很大程度上是未知的。为了确定Nef对翻译的影响，我们使用体外翻译测定。用免疫共沉淀法和RT-PCR法检测Nef/RPS 10复合物及复合物中18 S rRNA和tRNA的存在。我们观察到HIV-1 Nef蛋白在体外特异性损害翻译。用免疫共沉淀法观察Nef与RPS 10的相互作用。此外，18 S rRNA和tRNA存在于Nef/RPS 10复合物中。我们的研究结果与Nef蛋白通过与40 S小核糖体亚基RPS 10和18 S rRNA的两种组分结合，并在较小程度上与tRNA结合，可能导致蛋白质合成减少的模型一致。
Human immunodeficiency virus type 1 (HIV-1) Nef-encoded protein plays key functions at almost all stages of the viral life cycle, but its role in translation is largely unknown. To determine the effect of Nef on translation we used an in vitro translation assay. The detection of Nef/RPS10 complexes and the presence of 18S rRNA and tRNAs in the complexes were performed by coimmunoprecipitation and RT-PCR assay. We observed that the HIV-1 Nef protein specifically impaired translation in vitro. We observed the interaction of Nef with RPS10 by coimmunoprecipitation assay. In addition 18S rRNA and tRNAs were present in the Nef/RPS10 complexes. Our results are consistent with a model in which the Nef protein by binding to two components of the 40S small ribosomal subunit, RPS10 and 18S rRNA, and to a lesser extent to tRNAs, could lead to decreased protein synthesis.