The interactive effects of hepatic lipase gene promoter polymorphisms with sex and obesity on high-density-lipoprotein cholesterol levels in Taiwanese-Chinese
The interactive effects of hepatic lipase gene promoter polymorphisms with sex and obesity on high-density-lipoprotein cholesterol levels in Taiwanese-Chinese
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DOI：
10.1016/j.atherosclerosis.2003.09.013
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发表时间：
2004-01-01
期刊：
影响因子：
5.3
通讯作者：
Lee, YS
中科院分区：
文献类型：
--
作者：
Ko, YL;Hsu, LA;Lee, YS
目的：肝脂肪酶（HL）参与多种脂蛋白的代谢，并在胆固醇反向转运中发挥关键作用。本研究的目的是测试中国台湾人群中两个 HL 基因启动子多态性（HL-514C/T 和 HL-250G/A）与脂蛋白谱之间的统计关联。方法：对 716 名台湾华人样本群体进行了分析。从血液中提取DNA，并通过聚合酶链式反应、限制性酶消化和琼脂糖凝胶电泳确定基因型。结果：数据分析显示，这两个多态性存在强连锁不平衡（D/D-max = 0.97，P < 0.001）。与非携带者相比，携带 -514T 和 -250A 等位基因的携带者的总胆固醇/HDL-C 比率显着降低（P 分别为 0.007 和 0.004）。与非携带者相比，-514T 和 -250A 等位基因携带者的高密度脂蛋白胆固醇 (HDL-C) 水平也较高，也发现了这种关联的显着趋势（P 分别为 0.030 和 0.023）。多变量分析表明，HL-514C/T和HL-250G/A多态性对HDL-C水平的影响不受受试者性别、体重指数、血浆甘油三酯水平和胆固醇酯转移蛋白基因Taq1B多态性的影响。对每个性别的亚组分析表明，所研究的两个多态性与男性中的 HDL-C 水平显着相关，但在女性中则不显着。肥胖男性和非肥胖男性之间的相同关联并不一致。肥胖男性中 HDL-C 水平各自多态性的 P 值分别为 0.012 和 0.002，但在非肥胖男性中不显着。结论：我们的数据分析显示，台湾华人的 HL 基因启动子多态性与 HDL-C 水平之间存在独立关联。数据还表明，HL-514C/T 和 HL-250G/A 多态性与 HDL-C 水平上的性别和肥胖相互作用。研究结果为预防医学和临床诊断中识别高危人群提供了线索。随后对治疗情况和预后的影响来自这项研究。 (C) 2003 Elsevier Ireland Ltd. 保留所有权利。
Objectives: Hepatic lipase (HL) is involved in the metabolism of several lipoproteins and plays a key role in reverse cholesterol transport. The aim of the current study was to test the statistical association between two HL gene promoter polymorphisms (HL-514C/T and HL-250G/A) and lipoprotein profiles in a Taiwanese-Chinese population. Methods: A sample population of 716 Taiwanese-Chinese individuals was analyzed. DNA was extracted from the blood and genotypes were determined by polymerase chain reaction, restriction enzyme digestion, and agarose gel electrophoresis. Results: Analysis of the data revealed that these two polymorphisms are in strong linkage disequiliblium (D/D-max = 0.97. P < 0.001). A significantly lower total cholesterol/HDL-C ratio was noted for carriers with the -514T and -250A alleles compared to non-carriers (P = 0.007 and 0.004, respectively). A significant trend of the association was also found on the high levels of high-density-lipoprotein cholesterol (HDL-C) among carriers with the -514T and -250A alleles as opposed to that of non-carriers (P = 0.030 and 0.023. respectively). Multivariate analysis has demonstrated that the effects of HL-514C/T and HL-250G/A polymorphisms on HDL-C levels were not affected by subjects' sex, body mass index, plasma triglyceride levels and the cholesterol ester transfer protein gene Taq1B polymorphism. Subgroup analysis on each sex has revealed that the two studied polymorphisms were significantly associated with HDL-C levels among males but not significant in women. The same association between obese and non-obese men was not consistent. The P-value of the respective polymorphisms on HDL-C levels were 0.012 and 0.002 among obese men, but not significant among non-obese men. Conclusion: Analysis of our data revealed an independent association between the HL gene promoter polymorphisms and HDL-C levels in Taiwanese-Chinese. The data also suggests that the HL-514C/T and HL-250G/A polymorphisms interact with sex and obesity on HDL-C levels. The findings give clues for identifying high risk population in preventive medicine and clinical diagnosis. The subsequent impacts on treatment profiles and prognosis were derived from this study. (C) 2003 Elsevier Ireland Ltd. All rights reserved.