RESPONSE OF PORCINE AORTIC SMOOTH-MUSCLE CELLS TO CYCLIC TENSIONAL DEFORMATION IN CULTURE
RESPONSE OF PORCINE AORTIC SMOOTH-MUSCLE CELLS TO CYCLIC TENSIONAL DEFORMATION IN CULTURE
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DOI：
10.1016/0022-4804(88)90103-5
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发表时间：
1988-06-01
影响因子：
2.2
通讯作者：
BANES, AJ
中科院分区：
文献类型：
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作者：
SUMPIO, BE;BANES, AJ
血管壁持续承受脉动的流体静压。然而，大多数平滑肌细胞（SMC）的研究是在静态组织培养条件下进行的。我们利用一个灵活的底部培养板和施加周期性拉伸变形培养SMC。最近的研究表明，内皮细胞（EC）增殖，但保持随机取向时，受到3个周期/分钟的力方案高达24%的伸长率。目前的实验进行研究的影响，一个类似的力方案SMC。以200，000个细胞/35-mm 2孔接种猪SMC，并使其粘附24小时。24小时后，将培养板置于真空操作的应力提供装置上，该装置在培养板底部最大向下偏转时对培养板基底施加24%的伸长。使拉伸的细胞（n= 6威尔斯/天）经历10秒24%伸长和10秒松弛的循环7天。对照细胞（n= 6威尔斯/天）进行与对照组相似的孵育，但无循环变形。每24小时更换一次培养基。在收获前24小时，向平板中加入[3 H]胸苷（THY）（DNA合成的前体）（2 μCi/孔）。在第0、1、3、5和7天，对细胞进行计数并分析THY掺入。虽然以前的结果表明，脉动拉伸刺激EC，本研究表明，一个类似的方案的应力抑制SMC增殖。THY掺入SMC中抑制了观察到的细胞计数变化。在形态学上，SMC对齐在一个环形图案中施加的应变，而EC保持一个随机的方向。我们的结论是，脉动拉伸改变了培养中的细胞的行为，并对EC和SMC有相反的影响。
The vascular wall is continuously subjected to pulsatile hydrostatic pressures. However, most studies of smooth muscle cells (SMC) are done under static tissue culture conditions. We have utilized a flexible-bottom culture plate and applied cyclic tensional deformation to cultured SMC. Recent studies have shown that endothelial cells (EC) proliferate but remain randomly oriented when subjected to a force regimen of 3 cycle/min of up to 24% elongation. The current experiments were performed to study the effects of a similar force regimen on SMC. Porcine SMC were seeded at 200,000 cells/35-mm2well and permitted to adhere for 24 hr. After 24 hr, the plates were placed on a vacuum-operated stress-providing unit that exerted a 24% elongation on the culture plate substratum at maximum downward deflection of the plate bottom. The stretched cells (n= 6 wells/day) were subjected to cycles of 10 sec of 24% elongation and 10 sec of relaxation for 7 days. The control cells (n= 6 wells/day) were subjected to similar incubations as the control group but without cyclic deformation. Media were changed every 24 hr. [3H]Thymidine (THY), a precursor for DNA synthesis, was added (2 μCi/well) to plates 24 hr prior to harvesting. On Days 0, 1, 3, 5, and 7 cells were counted and analyzed for THY incorporation. While previous results indicate that pulsatile stretching stimulates EC, the present study indicates that a similar regimen of stress inhibits SMC proliferation. THY incorporation into SMC paralleled the observed changes in cell count. Morphologically, SMC aligned in an annular pattern in response to applied strain, whereas EC maintained a random orientation. We conclude that pulsatile stretching alters the behavior of cells in culture and has opposite effects on EC and SMC.