Visualizing Long-Term Memory Formation in Two Neurons of the Drosophila Brain
Visualizing Long-Term Memory Formation in Two Neurons of the Drosophila Brain
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DOI：
10.1126/science.1212735
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发表时间：
2012-02-10
期刊：
影响因子：
56.9
通讯作者：
Chiang, Ann-Shyn
中科院分区：
文献类型：
--
作者：
Chen, Chun-Chao;Wu, Jie-Kai;Chiang, Ann-Shyn
长期记忆（LTM）依赖于新蛋白质的合成。使用温度敏感的核糖体失活毒素，以急性抑制蛋白质的合成，我们筛选个别神经元，使新的蛋白质后，在果蝇的嗅觉联想条件反射。令人惊讶的是，在抑制两个背前外侧（DAL）神经元的蛋白质合成后，LTM受损，但在被认为是成人学习和记忆中心的蘑菇体（MB）中却没有。使用光转换荧光蛋白KAEDE报告从头蛋白质合成，我们已经直接可视化环磷酸腺苷（cAMP）反应元件结合蛋白（CREB）依赖的转录激活钙/钙调蛋白依赖性蛋白激酶II和周期基因的DAL神经元间隔后，但不集中的训练。在提取过程中阻断DAL中的神经输出会损害记忆保持，但在获取或巩固过程中不会。这些发现表明果蝇中存在额外的MB记忆回路：LTM巩固（MB到DAL），存储（DAL）和检索（DAL到MB）。
Long-term memory (LTM) depends on the synthesis of new proteins. Using a temperature-sensitive ribosome-inactivating toxin to acutely inhibit protein synthesis, we screened individual neurons making new proteins after olfactory associative conditioning in Drosophila. Surprisingly, LTM was impaired after inhibiting protein synthesis in two dorsal-anterior-lateral (DAL) neurons but not in the mushroom body (MB), which is considered the adult learning and memory center. Using a photoconvertible fluorescent protein KAEDE to report de novo protein synthesis, we have directly visualized cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB)-dependent transcriptional activation of calcium/calmodulin-dependent protein kinase II and period genes in the DAL neurons after spaced but not massed training. Memory retention was impaired by blocking neural output in DAL during retrieval but not during acquisition or consolidation. These findings suggest an extra-MB memory circuit in Drosophila: LTM consolidation (MB to DAL), storage (DAL), and retrieval (DAL to MB).