Effects of 25-hydroxyvitamin D3 on growth performance, serum parameters, fecal microbiota and metabolites in weaned piglets fed diets with low calcium and phosphorus.
Effects of 25-hydroxyvitamin D3 on growth performance, serum parameters, fecal microbiota and metabolites in weaned piglets fed diets with low calcium and phosphorus.
复制标题
DOI：
10.1002/jsfa.11388
复制
发表时间：
2021-06
影响因子：
4.1
通讯作者：
Lianhua Zhang;Mingrui Yang;X. Piao
中科院分区：
文献类型：
--
作者：
Lianhua Zhang;Mingrui Yang;X. Piao
背景随着集约化养殖的发展，猪长期暴露于弱光环境不利于维生素D3的产生，维生素D3缺乏会影响钙（Ca）、磷（P）的吸收和代谢。25-羟基维生素D3（25OHD3）比普通维生素D3具有更高的生物活性。本试验旨在研究25OHD 3对低钙磷日粮断奶仔猪生产性能、血清生化指标、粪便微生物及代谢产物的影响。结果NC +25-D提高试验2期和整个试验期的平均日增重（ADG）显著高于对照组（P <0.05），第28天血清免疫球蛋白G（IgG）、免疫球蛋白A（IgA）、过氧化氢酶（CAT）、骨特异性碱性磷酸酶（BALP）和骨钙素（OC）含量与对照组比较差异有显著性（P <0.05）;而PC组与NC +25-D组之间无显著性差异。与NC相比，PC和NC +25-D中厚壁菌门的丰度较高（P <0.05）。NC +25-D使链球菌科细菌的丰度较PC和NC降低（P <0.05），毛螺菌科细菌的丰度较NC升高（P <0.05）。血清25OHD 3与粪链球菌科菌数呈负相关（P <0.05），与粪毛螺菌科菌数呈正相关（P <0.05）。结论低钙磷日粮中添加25 OHD3可提高仔猪的血清免疫力、骨生化指标和粪便微生物群，降低链球菌科细菌数量，增加毛螺菌科细菌数量，促进仔猪生长，其效果与正常钙磷日粮相似。本文受版权保护。All rights reserved.
BACKGROUND With the development of intensive farming, long-term exposure of pigs to poor light conditions is not conducive to the production of vitamin D3 , and vitamin D3 deficiency could affect absorption and metabolism of calcium (Ca) and phosphorus (P). 25-hydroxyvitamin D3 (25OHD3 ) has higher bioactivity than regular vitamin D3 . This study was to investigate the effects of 25OHD3 on performance, serum parameters, fecal microbiota and metabolites in weaned piglets fed with low Ca-P diet. RESULTS NC + 25-D improved (P < 0.05) average daily gain (ADG) in phase 2 and overall period, as well as increased (P < 0.05) serum contents of immunoglobulin G (IgG), immunoglobulin A (IgA), catalase (CAT), bone-specific alkaline phosphatase (BALP) and osteocalcin (OC) on day 28 compared with NC, but no differences were observed between PC and NC + 25-D. Compared with NC, the abundance of Firmicutes was higher (P < 0.05) in PC and NC + 25-D. NC + 25-D decreased (P < 0.05) the abundance of Streptococcaceae compared with PC and NC, and increased (P < 0.05) the abundance of Lachnospiraceae compared with NC. Serum 25OHD3 was negatively correlated with the abundance of fecal Streptococcaceae (P < 0.05), and positively correlated with the abundance of fecal Lachnospiraceae (P < 0.05). CONCLUSION Supplementation of 25OHD3 in low Ca-P diet improved serum immunity, bone biochemical parameters and fecal microbiota such as decreased Streptococcaceae abundance and increased Lachnospiraceae abundance, which could subsequently promote growth of piglets, and the effects were similar to that of normal Ca-P diet. This article is protected by copyright. All rights reserved.