Robo functions as an attractive cue for glial migration through SYG-1/Neph.
Robo functions as an attractive cue for glial migration through SYG-1/Neph.
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作者：
Qu Z;Zhang A;Yan D
Robo是目前研究最多的受体之一，在许多生物学过程中发挥作用，其功能高度依赖于Robo的配体Slit。在这里，我们发现了一个狭缝独立的作用，Robo在胶质细胞迁移，并表明，神经元可以释放细胞外片段的Robo分裂吸引胶质细胞迁移过程中的秀丽隐杆线虫。此外，我们确定了保守的细胞粘附分子SYG-1/Neph作为切割的细胞外Robo片段的受体，通过调节WAVE复合物介导胶质细胞迁移和SYG-1/Neph功能。我们的研究揭示了以前未知的Robo的Slit独立功能和调节机制，并表明Robo的切割细胞外片段可以作为SYG-1/Neph的配体来引导胶质细胞迁移。由于Robo，Robo的切割区域和SYG-1/Neph在动物界都是高度保守的，我们的研究结果可能在大脑发育过程中呈现保守的Slit独立的Robo机制。
As one of the most-studied receptors, Robo plays functions in many biological processes, and its functions highly depend on Slit, the ligand of Robo. Here we uncover a Slit-independent role of Robo in glial migration and show that neurons can release an extracellular fragment of Robo upon cleavage to attract glia during migration in Caenorhabditis elegans. Furthermore, we identified the conserved cell adhesion molecule SYG-1/Neph as a receptor for the cleaved extracellular Robo fragment to mediate glial migration and SYG-1/Neph functions through regulation of the WAVE complex. Our studies reveal a previously unknown Slit-independent function and regulatory mechanism of Robo and show that the cleaved extracellular fragment of Robo can function as a ligand for SYG-1/Neph to guide glial migration. As Robo, the cleaved region of Robo, and SYG-1/Neph are all highly conserved across the animal kingdom, our findings may present a conserved Slit-independent Robo mechanism during brain development.