Potential of electrospray mass spectrometry for quantifying glycohemoglobin.
Potential of electrospray mass spectrometry for quantifying glycohemoglobin.
复制标题
电喷雾质谱法定量糖血红蛋白的潜力。
作者：
Norman B. Roberts;Brian N. Green;Michael A. Morris
建立了一种测定糖化血红蛋白的电喷雾质谱法。将来自78名糖尿病患者和50名非糖尿病患者的全血样品（糖化范围3- 15%，通过电喷雾质谱法测定）在酸性变性溶剂中稀释500倍，并直接引入质谱仪中。然后处理所得质谱以估计样品中存在的糖化血红蛋白的百分比。总分析时间（包括绘制光谱和计算糖化百分比）约为3 min。对于总糖化血红蛋白（范围为3.6- 14%）的批间和批内分析，该方法的不精密度（CV）< 5.1%。质谱结果与已建立的亲和色谱程序的比较显示出良好的总体一致性。直接测定α链和β链的相对糖基化，并在测量的糖基化范围内保持恒定（0.64：1）。仅观察到单个葡萄糖附着于α链和β链。
An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed to estimate the percentage of glycohemoglobin present in the sample. Total analysis time, including plotting the spectra and computing the percentage of glycation, was approximately 3 min. The imprecision (CV) of the method was < 5.1% for inter- and intrabatch analyses for total glycohemoglobin in the range 3.6-14%. Comparison of the mass spectrometric results with those from established affinity chromatographic procedures showed good overall agreement. The relative glycation of the alpha- and beta-chains was determined directly and was shown to be constant (0.64:1) over the glycation range measured. Only single glucose attachment to both the alpha- and beta-chains was observed.
糖化蛋白更新：最近研究的影响，包括糖尿病控制和并发症试验。
影响因子：
9.3
作者：
Benjamin,RJ;Sacks,DB
通讯作者：
Sacks,DB