Immunocytochemical localization of opsin in the cell membrane of developing rat retinal photoreceptors.
Immunocytochemical localization of opsin in the cell membrane of developing rat retinal photoreceptors.
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DOI：
10.1083/jcb.98.5.1788
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发表时间：
1984-05
期刊：
影响因子：
--
通讯作者：
Papermaster DS
中科院分区：
文献类型：
--
作者：
Nir I;Cohen D;Papermaster DS
成熟的视网膜视杆光感受器将视蛋白隔离在视杆外段（ROS）的视盘和质膜中。视蛋白在内节合成，并沿连接两个隔室的连接纤毛沿着转移到外节。我们研究了视网膜发育的早期阶段，在此期间视蛋白的极化分布在视杆细胞中建立。从3-21日龄的新生大鼠中分离视网膜，并与亲和纯化的生物素基-绵羊抗牛视蛋白，随后与抗生物素蛋白-铁蛋白一起孵育。在ROS发育之前的出生后早期，视蛋白被内节质膜上的抗视蛋白标记。在出生后第五天，随着ROS形成开始，在连接纤毛质膜上检测到视蛋白。然而，纤毛质膜的标记密度是不均匀的：近端纤毛是相对未标记的远端纤毛和ROS质膜相比。在接近成熟的大鼠视网膜，视蛋白不再检测到内段质膜。在用相同抗体标记的成人视杆细胞中也观察到类似的视蛋白极化分布。这些结果表明，连接纤毛及其质膜的某些成分可能参与了视蛋白极化分布的建立和维持。
Mature retinal rod photoreceptors sequester opsin in the disk and plasma membranes of the rod outer segment (ROS). Opsin is synthesized in the inner segment and is transferred to the outer segment along the connecting cilium that joins the two compartments. We have investigated early stages of retinal development during which the polarized distribution of opsin is established in the rod photoreceptor cell. Retinas were isolated from newborn rats, 3-21 d old, and incubated with affinity purified biotinyl-sheep anti-bovine opsin followed by avidin- ferritin. At early postnatal ages prior to the development of the ROS, opsin is labeled by antiopsin on the inner segment plasma membrane. At the fifth postnatal day, as ROS formation begins opsin was detected on the connecting cilium plasma membrane. However, the labeling density of the ciliary plasma membrane was not uniform: the proximal cilium was relatively unlabeled in comparison with the distal cilium and the ROS plasma membrane. In nearly mature rat retinas, opsin was no longer detected on the inner segment plasma membrane. A similar polarized distribution of opsin was also observed in adult human rod photoreceptor cells labeled with the same antibodies. These results suggest that some component(s) of the connecting cilium and its plasma membrane may participate in establishing and maintaining the polarized distribution of opsin.