ISOCITRATE DEHYDROGENASE AND RELATED OXIDATIVE DECARBOXYLASES
ISOCITRATE DEHYDROGENASE AND RELATED OXIDATIVE DECARBOXYLASES
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DOI：
10.1016/0014-5793(80)80569-2
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发表时间：
1980-01-01
期刊：
影响因子：
3.5
通讯作者：
DALZIEL, K
中科院分区：
文献类型：
--
作者：
DALZIEL, K
“中间代谢的研究通常在两个层次上进行。在第一个层次上，目的是确定来自底物分子的中间化合物。当中间体被发现时，就可以进入第二个层次--研究与中间体的活化有关的酶。这是HA Krebs的一篇历史性文章“碳水化合物生物氧化的中间阶段”的开头段落。它发表于1943年，在氧化代谢研究的两个水平上取得了十年的巨大进展，其中包括黄素和烟酰胺核苷酸酶辅因子的鉴定，柠檬酸循环的形成以及新酶的鉴定和研究，包括NADP连接的异柠檬酸脱氢酶。本文主要关注的是后者的酶（EC 1.1）的一些选定的“第二级”研究。1.42）、苹果酸酶（EC 1. L. 1.40）和6-磷酸-葡萄糖酸脱氢酶（EC 1. L. 1.44）。这些是催化一般类型的可逆反应的最已知的β-羟基酸脱羧酶：
‘The study of intermediary metabolism usually proceeds at two levels. At the first level the aim is to identify the intermediary compounds derived from the substrate molecule. When the intermediates have been discovered the second level can be approached-the study of the enzymes concerned with the activation of the intermediates.’This is the opening paragraph of an historic article on ‘The Intermediary Stages in the Biological Oxidation of Carbohydrates’ by HA Krebs [11. It was published in 1943, after a decade of dramatic advances at both levels of the study of oxidative metabolism, amongst which were the identification of flavin and nicotinamide nucleotide enzyme cofactors, the formulation of the citric acid cycle and the identification and study of new enzymes, including NADP-linked isocitrate dehydrogenase. The present article is primarily concerned with some selected ‘second level’studies of the latter enzyme (EC 1.1. 1.42), malic enzyme (EC 1. l. 1.40) and 6-phospho-gluconate dehydrogenase (EC 1. l. 1.44). These are the best known of the fi-hydroxyacid decarboxylases which catalyse reversible reactions of the general type: