Infections of granulocytic ehrlichiae and Borrelia burgdorferi in white-tailed deer in Connecticut
Infections of granulocytic ehrlichiae and Borrelia burgdorferi in white-tailed deer in Connecticut
复制标题
DOI：
10.7589/0090-3558-35.2.266
复制
发表时间：
1999-04-01
影响因子：
1.3
通讯作者：
Fikrig, E
中科院分区：
文献类型：
--
作者：
Magnarelli, LA;Ijdo, JW;Fikrig, E
对1980年、1991年和1996年在美国康涅狄格州采集的141只白尾鹿（Odocoileus virginianus）的血清或全血进行了分析，以检测过去或现在感染的嗜吞噬细胞埃利希体基因群生物和伯氏疏螺旋体。分别对BDS和NCH-1粒细胞埃希体进行间接荧光抗体（IFA）染色，1991年和1996年抗体阳性率分别为25% ~ 64%。1980年收集的50份血清检测结果均为阴性。尽管通过酶联免疫吸附试验检测到的含有伯氏疏螺旋体抗体的血清百分比也有所不同（23%至53%），但41份血清中有20份（49%）存在两种细菌的共存抗体。在特异性试验中，用IFA染色法检测了19例含有埃氏体抗体的鹿血清的边缘无原体抗体；一种对埃氏抗原滴度为1:5 120的血清在血清稀释度为1:20 20时与边缘a抗原反应。在69份血清的平行分析中，Western blot分析的结果与含有埃利希抗原的IFA染色方法的结果一致（72%一致）。所有阳性免疫印迹均显示人粒细胞埃利希体病（HGE）病原体NCH-1株的肽带，分子量约为44、105或110 kDa。对63只鹿的血液样本进行聚合酶链反应（PCR）研究，11份（18%）样本检测出嗜利希菌基因群生物16S核糖体DNA阳性，23份（37%）样本检测出嗜利希菌基因群生物44kda基因DNA阳性。白尾鹿在肩胛骨棒丰富的地区暴露于不同的蜱传细菌，在某些情况下可能同时感染。
Serum or whole blood samples, obtained from 141 white-tailed deer (Odocoileus virginianus) in Connecticut (USA) during 1980, 1991, and 1996, were analyzed to detect past or current infections of Ehrlichia phagocytophila genogroup organisms and Borrelia burgdorferi. When the BDS or NCH-1 strains of granulocytic ehrlichiae were used separately in indirect fluorescent antibody (IFA) staining methods, antibody positivity rates varied from 25 to 64% in 1991 and 1996, respectively. All 50 sera tested from 1980 collections were negative. Although percentages of sera with B. burgdorferi antibodies, as detected by an enzyme-linked immunosorbent assay, also differed (23 to 53%), there were coexisting antibodies to both bacteria in 20 (49%) of 41 sera. In tests on specificity, 19 deer sera with ehrlichial antibodies also were tested by IFA staining procedures for Anaplasma marginale antibodies; one serum with a titer of 1: 5,120 to ehrlichial antigen reacted to A. marginale antigen at a serum dilution of 1:320. In parallel analyses of 69 sera, results of Western blot analyses for ehrlichial infections in deer were concordant (72% agreement) with those of IFA staining methods containing ehrlichial antigen. All positive immunoblots showed bands to peptides of the NCH-1 strain of the human granulocytic ehrlichiosis (HGE) agent having molecular masses of about 44, 105, or 110 kDa. In polymerase chain reaction (PCR) studies of blood samples from 63 deer, 11(18%) specimens were positive for 16S ribosomal DNA of an Ehrlichia phagocytophila genogroup organism, whereas 23 (37%) samples were positive for the DNA of the 44 kDa gene of the HGE agent. White-tailed deer are exposed to different tick-borne bacteria in areas where bodes scapularis sticks are abundant and may, in some instances, have had concurrent infections.