RIBOSOMAL-RNA SEQUENCES OF SARCOCYSTIS-MURIS, THEILERIA-ANNULATA AND CRYPTHECODINIUM-COHNII REVEAL EVOLUTIONARY RELATIONSHIPS AMONG APICOMPLEXANS, DINOFLAGELLATES, AND CILIATES
RIBOSOMAL-RNA SEQUENCES OF SARCOCYSTIS-MURIS, THEILERIA-ANNULATA AND CRYPTHECODINIUM-COHNII REVEAL EVOLUTIONARY RELATIONSHIPS AMONG APICOMPLEXANS, DINOFLAGELLATES, AND CILIATES
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DOI：
10.1016/0166-6851(91)90036-6
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发表时间：
1991-03-01
影响因子：
1.5
通讯作者：
SOGIN, ML
中科院分区：
文献类型：
--
作者：
GAJADHAR, AA;MARQUARDT, WC;SOGIN, ML
鼠肉孢子虫是一种具有两个宿主生命周期的球虫，涉及家猫和小鼠（Mus musculus）。鼠链球菌和环泰勒虫属于顶复门，但后者是梨原体亚纲中蜱传的原虫，可引起牛的热带泰勒虫病。 使用聚合酶链式反应技术扩增这些生物体以及自由生活的甲藻隐甲藻的小亚基核糖体 RNA（16S 样 rRNA）编码区，并与其他真核生物的 16S 样 rRNA 序列进行比较。 S. muris 和 T. annulata 的 16S 样 rRNA 基因彼此之间的相似度高于恶性疟原虫（人类恶性三日疟的病因）或伯氏疟原虫（通常研究的啮齿动物疟疾的病原体）。 从 rRNA 序列相似性推断出的进化树支持以 Prorocentrum micans 和 C. cohnii 为代表的顶复门和甲藻之间存在密切的系统发育关系。 显然，这些相关门的成员源自产生纤毛原生动物的祖先。
Sarcocystis muris is a coccidium with a two-host life cycle involving the domestic cat and the mouse, Mus musculus. S. muris and Theileria annulata belong to the phylum Apicomplexa, but the latter organism is a tick-borne protozoon in the subclass Piroplasmea and causes tropical theileriosis in cattle. The small-subunit ribosomal RNA (16S-like rRNA) coding regions of these organisms as well as that of the free living dinoflagellate Crypthecodinium cohnii were amplified using polymerase chain reaction techniques and compared to 16S-like rRNA sequences from other eukaryotes. The 16S-like rRNA genes of S. muris and T. annulata are more similar to each other than either is to Plasmodium falciparum, the cause of malignant tertian malaria of humans or Plasmodium berghei, the agent of the commonly studied malaria of rodents. Evolutionary trees inferred from the rRNA sequence similarities support a close phylogenetic relationship between the Apicomplexa and Dinoflagellata as represented by Prorocentrum micans and C. cohnii. Apparently members of these related phyla arose from an ancestral stock that gave rise to the ciliated protozoa.