EXPRESSION OF SPARC/OSTEONECTIN TRANSCRIPT IN MURINE EMBRYOS AND GONADS
EXPRESSION OF SPARC/OSTEONECTIN TRANSCRIPT IN MURINE EMBRYOS AND GONADS
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DOI：
10.1111/j.1432-0436.1988.tb00792.x
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发表时间：
1988-01-01
期刊：
影响因子：
2.9
通讯作者：
STRICKLAND, S
中科院分区：
文献类型：
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作者：
HOWE, CC;OVERTON, GC;STRICKLAND, S
从小鼠畸胎癌来源的顶叶内胚样细胞中分离到一个cdna克隆p2-4，用于分析相应的转录本在小鼠胚胎发生过程中的表达。核苷酸序列分析表明，该克隆与从小鼠顶骨内胚层和牛骨细胞中克隆的SPARC/Osteonectin基因具有广泛的同源性。当胚胎癌细胞分化为壁内胚层样细胞时，SPARC/Osteonectin转录本更加丰富。在胚胎中，转录本在第11天开始出现在胚胎中，并在整个妊娠期继续表达。从第9天和第11天起，该转录物也分别存在于胚胎外膜和胎盘中。因此，该转录物的表达在EC细胞分化和胚胎发育过程中受到调控。在成年小鼠中，包括睾丸在内的几个非骨组织也表达了该转录本。对生殖细胞缺陷小鼠的分析表明，睾丸的非生殖细胞成分表达了该转录本。对小鼠睾丸细胞系的分析进一步表明，该转录本在支持细胞和间质细胞中含量丰富。包裹着排卵的卵丘细胞也表达了高水平的转录本。
A cDNA clone, p2-4, was isolated from mouse teratocarcinoma-derived parietal endoderm-like cells and used to analyze expression of the corresponding transcript during mouse embryogenesis. Nucleotide-sequence analysis revealed extensive homology between this clone and SPARC/osteonectin cDNA cloned from mouse parietal endoderm and bovine bone cells. The SPARC/osteonectin transcript became more abundant when embryonal carcinoma (EC) cells differentiated into parietal endoderm-like cells. In embryos, the transcript began to appear in the embryo proper on day 11 and continued to be expressed throughout the gestation period. The transcript was also present in extraembryonic membranes and placenta from days 9 and 11 onward, respectively. Thus, expression of the transcript was regulated during differentiation of EC cells and during embryogenesis. In adult mice, several non-bone tissues, including testis, also expressed the transcript. Analysis of germ-cell-deficient mice indicated that non-germ-cell components of the testis expressed the transcript. Analysis of mouse testicular cell lines further suggested that the transcript was abundant in Sertoli cells and Leydig cells. Cumulus oophorus cells that envelope the ovulated egg also expressed high levels of the transcript.