Genome expression analysis of Anopheles gambiae:: Responses to injury, bacterial challenge, and malaria infection
Genome expression analysis of Anopheles gambiae:: Responses to injury, bacterial challenge, and malaria infection
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DOI：
10.1073/pnas.092274999
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发表时间：
2002-06-25
影响因子：
11.1
通讯作者：
Kafatos, FC
中科院分区：
文献类型：
--
作者：
Dimopoulos, G;Christophides, GK;Kafatos, FC
冈比亚按蚊的微生物和疟疾的挑战，伤害，和氧化应激（在蚊子和/或培养的细胞系）的复杂的基因表达反应进行了调查，使用cDNA微阵列构建的EST克隆收集。表达谱被广泛细分为诱导和下调基因簇，革兰氏（+）和革兰氏（-）细菌和微生物激发子上调了一组不同的基因，许多属于免疫类，对疟疾的反应与这种反应部分重叠。氧化应激激活了一组独特的基因，主要涉及氧化还原过程。损伤上调和下调的基因簇也是独特的，分别显著涉及糖酵解相关基因和柠檬酸循环/氧化磷酸化/氧化还原线粒体功能。体内和体外反应的交叉比较表明存在可能对应于基因通路的紧密共调控基因组。
The complex gene expression responses of Anopheles gambiae to microbial and malaria challenges, injury, and oxidative stress (in the mosquito and/or a cultured cell line) were surveyed by using cDNA microarrays constructed from an EST-clone collection. The expression profiles were broadly subdivided into induced and down-regulated gene clusters, Gram(+) and Gram(-) bacteria and microbial elicitors up-regulated a diverse set of genes, many belonging to the immunity class, and the response to malaria partially overlapped with this response. Oxidative stress activated a distinctive set of genes, mainly implicated in oxicloreductive processes. Injury up- and down-regulated gene clusters also were distinctive, prominently implicating glycolysis-related genes and citric acid cycle/oxidative phosphorylation/redox-mitochondrial functions, respectively. Cross-comparison of in vivo and in vitro responses indicated the existence of tightly coregulated gene groups that may correspond to gene pathways.