P-ELEMENT HOMOLOGOUS SEQUENCES ARE TANDEMLY REPEATED IN THE GENOME OF DROSOPHILA-GUANCHE
P-ELEMENT HOMOLOGOUS SEQUENCES ARE TANDEMLY REPEATED IN THE GENOME OF DROSOPHILA-GUANCHE
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DOI：
10.1073/pnas.89.9.4018
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发表时间：
1992-05-01
影响因子：
11.1
通讯作者：
PINSKER, W
中科院分区：
文献类型：
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作者：
MILLER, WJ;HAGEMANN, S;PINSKER, W
在灌鼠果蝇中，p -同源序列位于单个基因组位点的串联重复阵列中（拷贝数为20-50）。通过原位杂交（O染色体：85C）确定多线染色体上的细胞学位置。一个完整重复单元（3.25千碱基）的测序结果显示，包含外显子0 ~ 2的中央编码区与果蝇P元件的相应部分序列相似性很高。序列的其余部分有很大的差异。外显子3没有编码功能，反向重复序列消失。番荔枝的P同源物显然失去了它们的移动性，但保留了一种类似于黑腹龙的66-kDa P元件抑制蛋白的编码能力。不同重复单位之间的差异表明该特定基因组位点的序列早期扩增。在亚bobscura、madeirensis和D. guanche中，在85℃存在一个共同的p元素位点，这表明该序列的聚类发生在这三个物种的系统发育辐射之前。
In Drosophila guanche, P-homologous sequences were found to be located in a tandem repetitive array (copy number: 20-50) at a single genomic site. The cytological position on the polytene chromosomes was determined by in situ hybridization (chromosome O: 85C). Sequencing of one complete repeat unit (3.25 kilobases) revealed high sequence similarity between the central coding region comprising exons 0 to 2 and the corresponding section of the Drosophila melanogaster P element. The rest of the sequence has diverged considerably. Exon 3 has no coding function and the inverted repeats have disappeared. The P homologues of D. guanche apparently have lost their mobility but have retained the coding capacity for a protein similar to the 66-kDa P-element repressor of D. melanogaster. Divergence between different repeat units indicates early amplification of the sequence at this particular genomic site. The presence of a common P-element site at 85C in Drosophila subobscura, Drosophila madeirensis, and D. guanche suggests that clustering of the sequence at this location took place before the phylogenetic radiation of the three species.