A multi-modality platform to image stem cell graft survival in the naive and stroke-damaged mouse brain
A multi-modality platform to image stem cell graft survival in the naive and stroke-damaged mouse brain
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DOI：
10.1016/j.biomaterials.2013.11.085
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发表时间：
2014-02-01
期刊：
影响因子：
14
通讯作者：
Hoehn, Mathias
中科院分区：
文献类型：
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作者：
Boehm-Sturm, Philipp;Aswendt, Markus;Hoehn, Mathias
神经干细胞移植已被广泛研究用于治疗中风等脑部疾病。为了跟踪植入后细胞的定位和功能状态，非侵入性成像是必不可少的。因此，我们开发了一个综合的多模式平台，利用19F磁共振成像和生物发光成像相结合的方法，对移植物的定位、密度和存活进行体内成像。我们定量分析了健康和中风受损小鼠脑移植后前4周的细胞移植物存活率，并将移植物活力的发现与宿主的天然免疫反应相关联。多模式成像平台将有助于改善中风以外的情况下的细胞治疗，并为临床翻译获得不可或缺的信息。(C)2013年提交人。爱思唯尔有限公司出版。保留所有权利。
Neural stem cell implantations have been extensively investigated for treatment of brain diseases such as stroke. In order to follow the localization and functional status of cells after implantation noninvasive imaging is essential. Therefore, we developed a comprehensive multi-modality platform for in vivo imaging of graft localization, density, and survival using 19F magnetic resonance imaging in combination with bioluminescence imaging. We quantitatively analyzed cell graft survival over the first 4 weeks after transplantation in both healthy and stroke-damaged mouse brain and correlated our findings of graft vitality with the host innate immune response. The multi-modality imaging platform will help to improve cell therapy also in context other than stroke and to gain indispensable information for clinical translation. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.