MODE OF ACTION OF YEAST KILLER TOXINS - CHANNEL FORMATION IN LIPID BILAYER-MEMBRANES
MODE OF ACTION OF YEAST KILLER TOXINS - CHANNEL FORMATION IN LIPID BILAYER-MEMBRANES
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DOI：
10.1038/302709a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
KAGAN, BL
中科院分区：
文献类型：
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作者：
KAGAN, BL
酵母杀伤蛋白的毒性作用似乎涉及对敏感细胞质膜的选择性功能损伤。生理效应包括K+渗漏（参考文献1，2），抑制氨基酸的主动转运1，3和细胞通道酸化1，4。这些作用与某些细菌大肠杆菌素的作用惊人地相似，这些细菌大肠杆菌素先前已被证明在膜中形成通道5。提出的作用机制通常假设质膜中毒素引起的有限渗透性变化1 - 4，6，7。我们在这里报告的杀手毒素从酵母克鲁维毕赤酵母形成离子渗透通道的磷脂双层膜，我们提出，thein vitroelectrophysiological特性，这些通道占中毒细胞中观察到的病态效应。这项工作的初步介绍已在其他地方发表8。
The toxic action of yeast killer proteins seems to involve selective functional damage to the plasma membrane of the sensitive cell. Physiological effects include leakage of K+(refs 1, 2), inhibition of active transport of amino acids1,3and acidification of the cell interior1,4. These effects are strikingly similar to the effects of certain bacterial colicins which have been demonstrated previously to form channels in membranes5. Proposed mechanisms of action have usually postulated a limited permeability change induced by the toxin in the plasma membrane1–4,6,7. We report here that a killer toxin from the yeastPichia kluyveriforms ion-permeable channels in phospholipid bilayer membranes, and we propose that thein vitroelectrophysiological properties of these channels account for the morbid effects observed in intoxicated cells. A preliminary account of this work has appeared elsewhere8.