GmANN, a glutathione S-transferase-interacting annexin, is involved in high temperature and humidity tolerance and seed vigor formation in transgenic Arabidopsis
GmANN, a glutathione S-transferase-interacting annexin, is involved in high temperature and humidity tolerance and seed vigor formation in transgenic Arabidopsis
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DOI：
10.1007/s11240-019-01655-x
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发表时间：
2019-07
期刊：
影响因子：
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通讯作者：
Jiaping Wei;Yingzi Shen;Hai-hong Zhao;Xiaolin Liu;Yanfeng Jia;Xingwang Yu;Hao Ma
中科院分区：
文献类型：
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作者：
Jiaping Wei;Yingzi Shen;Hai-hong Zhao;Xiaolin Liu;Yanfeng Jia;Xingwang Yu;Hao Ma
植物膜联蛋白是具有多种功能的蛋白质，如结合钙离子和带负电荷的脂质。虽然它们在植物逆境反应中的重要性已被广泛报道，但它们在种子中的功能却很少被研究。在发育中的大豆种子中发现了一种推测的膜联蛋白[Glycine max(L.)]。在我们之前的比较蛋白质组学研究中。本研究通过序列分析确定其为大豆膜联蛋白GmANN。实时荧光定量PCR （qRT-PCR）分析表明，高温高湿（HTH）胁迫下，gmann的转录水平显著升高。此外，与野生型（WT）种子相比，转基因拟南芥种子在HTH胁迫下表现出更强的抗性和更高的种子活力。与WT植株相比，gmannov表达植株的过氧化物酶活性增加，脂质过氧化水平降低，活性氧释放水平降低。酵母双杂交和双分子荧光互补实验表明，GmANN与大豆谷胱甘肽s -转移酶（GmGST）相互作用。综上所述，GmANN可能参与了植物对HTH胁迫的响应，并参与了种子活力的形成。
Plant annexins are proteins with various functions, such as binding calcium ions and negatively charging lipids. Although their importance in plant stress responses has been widely reported, their function in seeds is rarely studied. A putative annexin was found in developing seed of soybean [Glycine max(L.) Merr] in our previous comparative proteomics study. In this study, it was identified as a soybean annexin, GmANN, through sequence analysis. Quantitative real time PCR (qRT-PCR) analysis indicated that the transcription levels ofGmANNwere increased significantly under high temperature and humidity (HTH) stress. Moreover,GmANN-transgenicArabidopsisseeds showed greater resistance and higher seed vitality under HTH stress in contrast to the wild type (WT) seeds.GmANNoverexpression plants showed increased peroxidase activities, decreased lipid peroxidation levels and decreased reactive oxygen species release levels compared to the WT plants. Yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that GmANN interacted with soybean glutathione S-transferase (GmGST). Taken together, our results implied that GmANN might take part in response to HTH stress and be involved in seed vigor formation in plant.