Differential expression of dehydrin in flower buds of two Japanese apricot cultivars requiring different chilling requirements for bud break.
Differential expression of dehydrin in flower buds of two Japanese apricot cultivars requiring different chilling requirements for bud break.
复制标题
DOI：
10.1093/treephys/26.12.1559
复制
发表时间：
2006-12
期刊：
影响因子：
4
通讯作者：
H. Yamane;Yuki Kashiwa;Eiko Kakehi;K. Yonemori;H. Mori;Kyohei Hayashi;K. Iwamoto;R. Tao;I. Kataoka
中科院分区：
文献类型：
--
作者：
H. Yamane;Yuki Kashiwa;Eiko Kakehi;K. Yonemori;H. Mori;Kyohei Hayashi;K. Iwamoto;R. Tao;I. Kataoka
在这项研究中，我们调查了日本杏（梅Siebold Zucc.）休眠花蕾蛋白质谱的季节变化。栽培品种'Ellching'，来自亚热带台湾，和'Nanko'，来自温带日本。一个蛋白质，分离出的二维聚丙烯酰胺凝胶电泳的花蕾提取物，肽测序显示是一个reflectin（组D-11莱亚（晚期胚胎发生丰富）蛋白质）。不同品种之间的蛋白质和转录本积累的模式不同，与更大的积累和更长的持续时间在“南科”比“Ellching”。这些差异与‘南果’比‘埃尔金’更需要低温来打破花芽休眠有关。我们的研究支持了早期工作的结果，比较了万年青和落叶桃（Prunus persica（L.）Batsch）基因型，并表明，在休眠季节的作用是共同的所有李属物种。
In this study, we investigated seasonal changes in protein profiles in dormant flower buds of Japanese apricot (Prunus mume Siebold Zucc.) cultivars 'Ellching', from subtropical Taiwan, and 'Nanko', from temperate Japan. One protein, isolated by two-dimensional polyacrylamide gel electrophoresis of flower bud extracts, was shown by peptide sequencing to be a dehydrin (the group of D-11 LEA (late embryogenesis-abundant) proteins). Patterns of dehydrin protein and transcript accumulation differed between the cultivars, with greater accumulations and longer persistence in 'Nanko' than in 'Ellching'. These differences correspond with the greater requirement for chilling to break flower bud dormancy in 'Nanko' than in 'Ellching'. Our study supports the findings of earlier work comparing dehydrin expression in the bark tissue of the evergreen and deciduous peach (Prunus persica (L.) Batsch) genotypes, and suggests that the role of dehydrin during the dormant season is common to all Prunus species.