DISTINCT PATHWAYS FOR AUTOCRINE AND PARACRINE WINGLESS SIGNALING IN DROSOPHILA EMBRYOS
DISTINCT PATHWAYS FOR AUTOCRINE AND PARACRINE WINGLESS SIGNALING IN DROSOPHILA EMBRYOS
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DOI：
10.1038/372461a0
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发表时间：
1994-12-01
期刊：
影响因子：
64.8
通讯作者：
HOOPER, JE
中科院分区：
文献类型：
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作者：
HOOPER, JE
两种分泌的蛋白质，Wingless(1，2)和Hedgehog(3，4)，指示果蝇胚胎分节表皮内的细胞命运(见参考文献)。5)。无翼(Wg)在每个副节段最后面的细胞中表达，Hedgehog(HH)在下一个副节段最前面的细胞中表达。原肠胚形成后，两种细胞类型立即相互依赖(6，7)。局部Wg信号稳定HH表达(8-10)，局部HH信号稳定Wg表达(11，12)。直接的WG自动调节(自分泌信号)被其维持HH的旁分泌作用所掩盖，而HH又维持WG。我使用了zust-White 3(Zw3)(13)和patted(Ptc)(11，14)突变背景来从基因上分离这个正反馈环，并研究自分泌Wg信号。我在此报告，WG直接自动调节不同于向相邻细胞发送WG信号，其重要性在于融合(FU)、平滑(SMO)和肘部中断(Ci)相对于zw3和Arm(Arm)。我还发现，在这个HH依赖的早期阶段，Wg自动调节不同于后来的Wg自动调节(15)，因为缺乏醋栗(GSB)参与。
Two secreted proteins, Wingless(1,2) and Hedgehog(3,4), instruct cell fates within the segmented epidermis of Drosophila embryos (reviewed in ref. 5). Wingless (Wg) is expressed by the most posterior cells in each parasegment; Hedgehog (Hh) is expressed in the most anterior cells of the next parasegment. Immediately after gastrulation, the two cell types are mutually dependent(6,7). Local Wg signalling stabilizes Hh expression(8-10) and local Hh signalling stabilizes Wg expression(11,12). Direct Wg autoregulation (autocrine signalling) is masked by its paracrine role in maintaining hh, which in turn maintains wg. I have used zeste-white3 (zw3)(13) and patched (ptc)(11,14) mutant backgrounds to uncouple genetically this positive-feedback loop and to study autocrine Wg signalling. I report here that direct Wg autoregulation differs from Wg signalling to adjacent cells in the importance of fused (fu), smoothened (smo) and cubitus interruptus (ci) relative to zw3 and armadillo (arm). I also find that Wg autoregulation during this early hh-dependent phase differs from later Wg autoregulation(15) by lack of gooseberry (gsb) participation.