Accumulation of FoxP3-expressing CD4+CD25+ T cells with distinct chemokine receptors in synovial fluid of patients with active rheumatoid arthritis
Accumulation of FoxP3-expressing CD4+CD25+ T cells with distinct chemokine receptors in synovial fluid of patients with active rheumatoid arthritis
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DOI：
10.1080/03009740701482800
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发表时间：
2007-11-01
影响因子：
2.1
通讯作者：
Wang, Y.
中科院分区：
文献类型：
--
作者：
Jiao, Z.;Wang, W.;Wang, Y.
目的：探讨活动期类风湿关节炎(RA)患者滑液中表达FoxP3的CD4(+)CD25(+)调节性T细胞的存在及其特点。方法：采用流式细胞术对活动期类风湿关节炎(RA)患者滑液中表达FoxP3的CD4(+)CD25(+)调节性T细胞和正常人外周血(PB)中表达FoxP3的CD4(+)CD25(+)调节性T细胞的频率和趋化因子受体表达谱进行检测。结果：RA患者外周血中表达FoxP3的CD4(+)CD25(+)FoxP3(+)T细胞的频率和趋化因子受体表达水平均显著高于正常对照组(P<0.05)。配对的外周血来自类风湿关节炎患者和来自健康对照组的外周血(p<；0.05)。然而，类风湿关节炎患者外周血中这一频率显著低于健康对照组外周血(p<；0.05)。值得注意的是，SF中的CD4(+)CD25(+)FoxP3(+)T细胞表达与炎症相关的趋化因子受体CCR4、CCR5和CXCR4。结论：RA SF中存在表达FoxP3的调节性T细胞的聚集，这种聚集可能依赖于调节性T细胞上表达的不同的趋化因子受体。
Objective: To explore the presence and characteristics of FoxP3-expressing CD4(+)CD25(+) regulatory T cells in synovial fluid (SF) of patients with active rheumatoid arthritis (RA).Methods: The frequency and chemokine receptors expression profile of FoxP3-expressing CD4(+)CD25(+) regulatory T cells in SF and peripheral blood (PB) from RA patients and PB from healthy controls were investigated by flow cytometry using three- or four-colour intracellular staining.Results: The frequency of CD4(+)CD25(+) FoxP3(+) T cells was increased significantly in SF compared with paired PB from RA patients and PB from healthy controls (p < 0.05). However, the frequency in PB from RA patients was significantly lower than in PB from healthy controls (p < 0.05). Notably, CD4(+)CD25(+)FoxP3(+) T cells in SF expressed increased levels of inflammation-related trafficking chemokine receptors, such as CCR4, CCR5, and CXCR4.Conclusion: There is an accumulation of FoxP3-expressing regulatory T cells in RA SF, and such recruitment may be dependent on the distinct chemokine receptors expressed on regulatory T cells.