Prevalence of Autoimmune Liver Disease Related Autoantibodies in Chinese Patients with Primary Biliary Cirrhosis
Prevalence of Autoimmune Liver Disease Related Autoantibodies in Chinese Patients with Primary Biliary Cirrhosis
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DOI：
10.1007/s10620-011-1756-1
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发表时间：
2011-11-01
影响因子：
3.1
通讯作者：
Li, Yongzhe
中科院分区：
文献类型：
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作者：
Hu, Chaojun;Deng, Chuiwen;Li, Yongzhe
背景：自身免疫性肝病（AiLD）相关自身抗体在中国原发性胆汁性肝硬化（PBC）患者中的患病率尚未明确，因此需要进行特征描述。目的本研究旨在探讨中国PBC患者中与aild相关的自身抗体的患病率。方法采用间接免疫荧光法检测198例PBC、44例自身免疫性肝炎（AIH）和41例非自身免疫性肝病（LDC）对照者血清中的抗线粒体抗体（AMA）。采用ELISA法检测抗m2、抗gp210、抗sp100、抗sla、抗lc1和抗lkm -1抗体的存在。结果在PBC、AIH和LDC患者中，AMA的发生率分别为92.4%、15.9%和7.3%。抗- m2在87.4%的PBC患者中存在，但在AIH和LDC患者中分别仅为4.5%和4.9%。抗gp210和抗sp100分别在34.3%和25.8%的PBC患者、6.8和15.9%的AIH患者以及4.9%和17.1%的LDC患者中检测到。抗lc1、抗sla和抗lkm -1分别存在于1.5%、0.0和0.5%的PBC中，存在于9.1、11.4和2.3%的AIH中，不存在于LDC中。ama阳性PBC患者IgM水平高于阴性PBC患者，CRP水平低于阴性PBC患者。抗gp210阳性的PBC患者ALT、TBIL、DBIL、GGT、ALP、AST水平较高，ALB水平较低（P < 0.05）。结论我们提供的与aild相关的自身抗体的流行率可能有助于确定中国的PBC。与抗gp210相比，抗sp100并不是中国PBC患者更好的预后指标。对于ama阴性PBC患者，仍然需要确定有效的诊断性生物标志物。
Background The prevalence of autoimmune liver disease (AiLD)-related autoantibodies has not been defined in Chinese patients with primary biliary cirrhosis (PBC) and therefore needs to be characterized.Aims The purpose of this study was to explore the prevalence of AiLD-related autoantibodies in Chinese patients with PBC.Methods Indirect immunofluorescence was used to detect anti-mitochondrial antibodies (AMA) in the sera from 198 PBC, 44 autoimmune hepatitis (AIH) and 41 non-autoimmune liver disease controls (LDC). ELISA assays were used to test the presence of anti-M2, anti-gp210, anti-sp100, anti-SLA, anti-LC1 and anti-LKM-1 antibodies.Results AMA was present in 92.4, 15.9 and 7.3%, respectively, of patients with PBC, AIH and LDC. Anti-M2 was present in 87.4% of the PBC patients, but only in 4.5 and 4.9%, respectively, of AIH and LDC patients. Anti-gp210 and anti-sp100 were detected in 34.3 and 25.8% of PBC, 6.8 and 15.9% of AIH and in 4.9 and 17.1% of LDC patients. Anti-LC1, anti-SLA and anti-LKM-1 were, respectively, present in 1.5, 0.0 and 0.5% of PBC, and 9.1, 11.4 and 2.3% of AIH, and none of LDC. PBC patients that were AMA-positive presented with higher IgM levels but lower CRP levels than negative ones. ALT, TBIL, DBIL, GGT, ALP, and AST were detected at higher levels and ALB at lower levels in PBC positive for anti-gp210 (P < 0.05).Conclusions The prevalence of AiLD-related autoantibodies we provided might help define PBC in China. Anti-sp100 is not a better prognostic marker for Chinese PBC patients compared to anti-gp210. The identification of effective diagnostic biomarkers for AMA-negative PBC patients is still needed.