Urinary ATP as an indicator of infection and inflammation of the urinary tract in patients with lower urinary tract symptoms.
Urinary ATP as an indicator of infection and inflammation of the urinary tract in patients with lower urinary tract symptoms.
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DOI：
10.1186/s12894-015-0001-1
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发表时间：
2015-02-21
期刊：
影响因子：
2
通讯作者：
Malone-Lee J
中科院分区：
文献类型：
--
作者：
Gill K;Horsley H;Kupelian AS;Baio G;De Iorio M;Sathiananamoorthy S;Khasriya R;Rohn JL;Wildman SS;Malone-Lee J
三磷酸腺苷（ATP）是一种神经递质和炎性细胞因子，与下尿路疾病的病理生理有关。ATP还反映了微生物生物量，因此有可能作为尿路感染（UTI）的替代标志物。ATP尿分析的最佳临床采样方法尚未确立。我们测试了尿ATP在评估下尿路症状、感染和炎症方面的潜力，并验证了用于临床实践的采样方法。在2009年10月至2012年10月期间，对出现下尿路症状（LUTS）的成人患者和无症状对照进行了一项前瞻性、设盲、横断面观察性研究。尿ATP的测定采用荧光素酶法，脓尿计数采用新鲜未离心尿液显微镜检查，症状评估采用有效的问卷。还验证了样品采集、储存和处理方法。对照组75例，LUTS组340例，分为无脓尿组（n = 100）、脓尿1-9 wbc μl-1组（n = 120）和脓尿≥10 wbc μl-1组（n = 120）。女性、排尿症状、脓尿> 10 wbc μl-1和MSU培养阴性者尿ATP升高。ROC曲线分析显示，没有证据表明诊断测试潜力。在23°C下储存时，尿ATP信号衰减，但在≤ -20 ° C下立即冷冻，不含硼酸防腐剂，冷冻前不需要离心尿液，可以防止尿ATP信号衰减。尿ATP可作为一种研究工具，但作为替代临床诊断标志物并不令人信服。
Adenosine-5′-triphosphate (ATP) is a neurotransmitter and inflammatory cytokine implicated in the pathophysiology of lower urinary tract disease. ATP additionally reflects microbial biomass thus has potential as a surrogate marker of urinary tract infection (UTI). The optimum clinical sampling method for ATP urinalysis has not been established. We tested the potential of urinary ATP in the assessment of lower urinary tract symptoms, infection and inflammation, and validated sampling methods for clinical practice. A prospective, blinded, cross-sectional observational study of adult patients presenting with lower urinary tract symptoms (LUTS) and asymptomatic controls, was conducted between October 2009 and October 2012. Urinary ATP was assayed by a luciferin-luciferase method, pyuria counted by microscopy of fresh unspun urine and symptoms assessed using validated questionnaires. The sample collection, storage and processing methods were also validated. 75 controls and 340 patients with LUTS were grouped as without pyuria (n = 100), pyuria 1-9 wbc μl-1 (n = 120) and pyuria ≥10 wbc μl-1 (n = 120). Urinary ATP was higher in association with female gender, voiding symptoms, pyuria greater than 10 wbc μl-1 and negative MSU culture. ROC curve analysis showed no evidence of diagnostic test potential. The urinary ATP signal decayed with storage at 23°C but was prevented by immediate freezing at ≤ -20°C, without boric acid preservative and without the need to centrifuge urine prior to freezing. Urinary ATP may have a role as a research tool but is unconvincing as a surrogate, clinical diagnostic marker.
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影响因子：
--
作者：
KASS, EH
通讯作者：
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影响因子：
6.6
作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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