Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta
Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta
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DOI：
10.1634/stemcells.2004-0058
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发表时间：
2004-01-01
期刊：
影响因子：
5.2
通讯作者：
Kanhai, HHH
中科院分区：
文献类型：
--
作者：
in't Anker, PS;Scherjon, SA;Kanhai, HHH
最近我们报道，中期妊娠羊水（AF）是胎儿间充质干细胞（MSC）的丰富来源。在这项研究中，我们分析了这些间充质干细胞的起源和存在的间充质干细胞在人类长期AF。此外，不同部位的人胎盘进行了研究，无论是胎儿或母体间充质干细胞的存在。我们比较了来源于房颤和胎盘的间充质干细胞的表型和生长特性。（平均胎龄，19（+2）[标准差，+/-1（+3）]周，n = 10）和足月妊娠晚期（平均胎龄，38（+4）[标准差，+/-1]周，n = 10）AF、羊膜、基底蜕膜，培养液中添加10%胎牛血清和内皮细胞生长因子。培养的细胞进行免疫表型特征，成脂和成骨分化能力进行了测试，并分析了生长动力学。胎儿和母体细胞的来源是通过人类白细胞抗原分子分型确定的。我们成功地从中期妊娠的AF，羊膜，和底蜕膜以及足月羊膜，壁蜕膜，和底蜕膜中分离出MSC。相比之下，MSC仅从10个晚期AF样品中的2个培养。从胎盘的不同胎儿和母体部分培养的MSC的表型是相当的。从中期妊娠和足月的基底蜕膜和顶蜕膜中提取的母体MSCs比成人骨髓中提取的MSCs表现出更高的扩增能力（p <0.05）。羊膜是胎儿间充质干细胞的新来源，可能有助于间充质干细胞在AF中的存在。底蜕膜和壁蜕膜是母体间充质干细胞的来源。与成人骨髓来源的MSC相比，胎儿和母体胎盘来源的MSC的扩增效力更高。
Recently we reported that second-trimester amniotic fluid (AF) is an abundant source of fetal mesenchymal stem cells (MSCs). In this study, we analyze the origin of these MSCs and the presence of MSCs in human-term AF. In addition, different parts of the human placenta were studied for the presence of either fetal or maternal MSCs. We compared the phenotype and growth characteristics of MSCs derived from AF and placenta.Cells from human second-trimester (mean gestational age, 19(+2) [standard deviation, +/-1(+3)] weeks, n = 10) and term third-trimester (mean gestational age, 38(+4) [standard deviation, +/-1] weeks, n = 10) AF, amnion, decidua basalis, and decidua parietalis were cultured in M199 medium supplemented with 10% fetal calf serum and endothelial cell growth factor. Cultured cells were immunophenotypically characterized, the adipogenic and osteogenic differentiation capacity was tested, and the growth kinetics were analyzed. The origin of fetal and maternal cells was determined by molecular human leukocyte antigen typing.We successfully isolated MSCs from second-trimester AF, amnion, and decidua basalis as well as term amnion, decidua parietalis, and decidua basalis. In contrast, MSCs were cultured from only 2 out of 10 term AF samples. The phenotype of MSCs cultured from different fetal and maternal parts of the placenta was comparable. Maternal MSCs from second-trimester and term decidua basalis and parietalis showed a significantly higher expansion capacity than that of MSCs from adult bone marrow (p < .05).Our results indicate that both fetal and maternal MSCs can be isolated from the human placenta. Amnion is a novel source of fetal MSCs, likely contributing to the presence of MSCs in AF. Decidua basalis and decidua parietalis are sources for maternal MSCs. The expansion potency from both fetal and maternal placenta-derived MSCs was higher compared with adult bone marrow-derived MSCs.