Stress-inducible expression of barley Hva1 gene in transgenic mulberry displays enhanced tolerance against drought, salinity and cold stress
Stress-inducible expression of barley Hva1 gene in transgenic mulberry displays enhanced tolerance against drought, salinity and cold stress
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DOI：
10.1007/s11248-011-9577-8
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发表时间：
2012-10-01
影响因子：
3
通讯作者：
Khurana, Paramjit
中科院分区：
文献类型：
--
作者：
Checker, Vibha G.;Chhibbar, Anju K.;Khurana, Paramjit
应对各种生物和非生物胁迫是可持续农业的基础。桑树的常规育种和分子标记辅助选择已被广泛应用。为了培育更好的品种，尽管这些方法所需的时间较长，因此必须使用精确的生物技术方法促进可持续农业。为了提高桑葚的抗逆性，利用农杆菌介导法将大麦胚胎发生晚期丰富基因HVA 1导入桑葚。在组成型启动子actin 1的调控下，转大麦Hva 1基因的桑葚表现出耐旱和耐盐的特性。在这里，我们报告，大麦Hva 1的过表达也赋予转基因桑葚的耐冷性。此外，在胁迫诱导型启动子rd 29 A控制下的大麦Hva 1基因可以有效地消除非胁迫条件下的生长迟缓，并赋予转基因桑葚胁迫耐受性。通过游离脯氨酸、膜稳定性指数和PSII活性测定，转基因株系表现出正常形态，生长增强，对干旱、盐和寒冷条件的耐受性增加。在胁迫条件下检测到蛋白质积累，证实了HVA 1在转基因中的诱导表达。在田间条件下评估这些植物的胁迫耐受性的研究显示了比非转基因植物更好的总体表现。胁迫响应基因如Mi dnaJ和Mi 2-cyperoxidin的表达增强表明Hva 1可以调节与提供非生物胁迫耐受性相关的下游基因。对转基因株系的研究表明，过量表达大麦Hva 1的植株具有耐旱、耐盐和耐冷的特性，表明拟南芥rd 29 A启动子在桑葚中具有一定的功能。
Coping with different kinds of biotic and abiotic stresses is the foundation of sustainable agriculture. Although conventional breeding and marker-assisted selection are being employed in mulberry (Morus indica L.) to develop better varieties, nonetheless the longer time periods required for these approaches necessitates the use of precise biotechnological approaches for sustainable agriculture. In an attempt to improve stress tolerance of mulberry, an important plant of the sericulture industry, an encoding late embryogenesis abundant gene from barley (HVA1) was introduced into mulberry plants by Agrobacterium-mediated transformation. Transgenic mulberry with barley Hva1 under a constitutive promoter actin1 was shown to enhance drought and salinity tolerance. Here, we report that overexpression of barley Hva1 also confers cold tolerance in transgenic mulberry. Further, barley Hva1 gene under control of a stress-inducible promoter rd29A can effectively negate growth retardation under non-stress conditions and confer stress tolerance in transgenic mulberry. Transgenic lines display normal morphology to enhanced growth and an increased tolerance against drought, salt and cold conditions as measured by free proline, membrane stability index and PSII activity. Protein accumulation was detected under stress conditions confirming inductive expression of HVA1 in transgenics. Investigations to assess stress tolerance of these plants under field conditions revealed an overall better performance than the non-transgenic plants. Enhanced expression of stress responsive genes such as Mi dnaJ and Mi 2-cysperoxidin suggests that Hva1 can regulate downstream genes associated with providing abiotic stress tolerance. The investigation of transgenic lines presented here demonstrates the acquisition of tolerance against drought, salt and cold stress in plants overexpressing barley Hva1, indicating that Arabidopsis rd29A promoter can function in mulberry.