Quantitative Oral HPV16 and HPV18 Detection in Persons Attending Dental Clinics.
Quantitative Oral HPV16 and HPV18 Detection in Persons Attending Dental Clinics.
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DOI：
10.1097/olq.0000000000001097
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发表时间：
2020-02
影响因子：
3.1
通讯作者：
Wald A
中科院分区：
文献类型：
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作者：
Stankiewicz Karita HC;Magaret A;Huang ML;Jerome KR;Feng Q;Wald A
本研究旨在评估定量人乳头瘤病毒（HPV）16型和HPV 18型检测口腔冲洗获得在西雅图，华盛顿牙科诊所。我们评估了2016年至2018年牙科就诊期间收集的口腔冲洗液。采用多重TaqMan定量聚合酶链反应检测HPV 16和HPV 18病毒载量（VL）。在15，313人中，152人（1%）可检测到口腔HPV 16/18。男性口腔HPV 16/18感染的风险高于女性（1.6% vs. 0.6%;比值比，3.2; 95%置信区间，2.1-4.4）。与女性相比，HPV 16男性年龄较大（中位数，55 vs. 48岁; P < 0.001），VL较高（39.7 vs. 1.1拷贝/mL，P < 0.001）。在39例基线时HPV 16和第二次口腔冲洗中，13例在随后的冲洗中保持阳性;在8例基线时HPV 18中，2例在随后的冲洗中保持阳性。连续阳性检测结果的人都是男性，与第一次阳性和第二次阴性样本的人相比，基线VL更高。口腔冲洗是HPV检测的一种可接受的方法，人们对检测感兴趣。HPV 16/18的总体患病率较低，男性的检出率高于女性，尤其是在高拷贝数时。HPV 16持续性在基线检测时VL高的男性中更常见。未来的研究需要评估的可行性，有效的二级预防策略口咽癌使用定量口腔HPV检测。
This study aimed to assess quantitative human papillomavirus (HPV) type 16 and HPV18 detection in oral rinses obtained in dental offices in Seattle, Washington. We evaluated oral rinses collected during dental visits from 2016 to 2018. Multiplex TaqMan quantitative polymerase chain reaction was used to determine HPV16 and HPV18 viral load (VL). Of 15,313 persons, 152 (1%) had detectable oral HPV16/18. Men were at higher risk of oral HPV16/18 infection than women (1.6% vs. 0.6%; odds ratio, 3.2; 95% confidence interval, 2.1–4.4). Compared with women, men with HPV16 were older (median, 55 vs. 48 years; P < 0.001) and had higher VL (39.7 vs. 1.1 copies/mL, P < 0.001). Of 39 with HPV16 at baseline and a second oral rinse, 13 remained positive at subsequent rinse; of 8 with HPV18 at baseline, 2 remained positive at subsequent rinse. Persons with consecutive positive test results were all men and had higher baseline VL compared with those with first positive and second negative samples. Oral rinse is an acceptable method of HPV testing, and persons are interested in testing. Overall HPV16/18 prevalence was low, and detection was more frequent among men than women, especially at higher copy numbers. HPV16 persistence was more common in men with high VL at baseline test. Future studies are needed to evaluate the feasibility of an effective secondary prevention strategy for oropharyngeal cancer using quantitative oral HPV detection.