Creation of human cardiac cell sheets using pluripotent stem cells
Creation of human cardiac cell sheets using pluripotent stem cells
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DOI：
10.1016/j.bbrc.2012.07.089
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发表时间：
2012-08-24
影响因子：
3.1
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Matsuura, Katsuhisa;Wada, Masanori;Okano, Teruo
尽管我们以前报道过新生大鼠心肌细胞膜通过反复移植血管形成致密增厚的心肌组织，但人心肌细胞膜的细胞来源及其功能尚未完全阐明。在这项研究中，我们开发了一种生物反应器来扩增和诱导人诱导多能干细胞(HiPSCs)向心脏分化。生物反应器培养14天，每100ml血管产生约8×10(7)个细胞，约80%的细胞心肌肌钙蛋白T阳性。心脏分化后，心肌细胞在温度敏感的培养皿中培养，即使在细胞片从培养皿中分离后，也表现出自发和同步的搏动。此外，当两个心肌细胞膜部分重叠时，观察到细胞外动作电位在细胞膜之间的传播。这些发现表明，由HiPSC来源的心肌细胞形成的心肌细胞膜可能具有足够的特性来产生增厚的心脏组织。(C)2012 Elsevier Inc.保留所有权利。
Although we previously reported the development of cell-dense thickened cardiac tissue by repeated transplantation-based vascularization of neonatal rat cardiac cell sheets, the cell sources for human cardiac cells sheets and their functions have not been fully elucidated. In this study, we developed a bioreactor to expand and induce cardiac differentiation of human induced pluripotent stem cells (hiPSCs). Bioreactor culture for 14 days produced around 8 x 10(7) cells/100 ml vessel and about 80% of cells were positive for cardiac troponin T. After cardiac differentiation, cardiomyocytes were cultured on temperature-responsive culture dishes and showed spontaneous and synchronous beating, even after cell sheets were detached from culture dishes. Furthermore, extracellular action potential propagation was observed between cell sheets when two cardiac cell sheets were partially overlaid. These findings suggest that cardiac cell sheets formed by hiPSC-derived cardiomyocytes might have sufficient properties for the creation of thickened cardiac tissue. (c) 2012 Elsevier Inc. All rights reserved.