Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil
Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil
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DOI：
10.1155/2017/4287547
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发表时间：
2017-01-01
影响因子：
3.4
通讯作者：
Moreira, Beatriz Meurer
中科院分区：
文献类型：
--
作者：
de Sousa, Viviane Santos;de Souza da-Silva, Ana Paula;Moreira, Beatriz Meurer
腐生葡萄球菌是年轻女性尿路感染(UTI)的重要病原体，但在人类微生物区系和一般环境中对该病原菌的研究较少。本研究的目的是从里约热内卢市621名孕妇、10个Minas奶酪包装和5个海滩的生殖器肛门微生物群中分离出腐生葡萄球菌的特征，并将这些菌株的PFGE图谱与该市描述的5个UTI PFGE聚类群进行比较。我们调查了65株来自微生物区系的腐生链球菌，13株来自米纳斯奶酪的腐生链球菌，30株来自海滩的腐生链球菌和32株UTI。药敏试验采用纸片扩散法，最低抑菌浓度测定采用琼脂稀释法和聚合酶链式反应。红霉素耐药基因erm(C)、msr(A)、msr(B)、mph(C)和lin(A)在93%的分离株中存在。甲氧苄氨嘧啶-磺胺甲恶唑耐药与dfrG或dfrA基因相关。3株头孢西丁耐药株携带mecA基因。从奶酪中分离到的所有菌株对所有抗菌剂都敏感。携带>；1分离株的10名孕妇中有6名有单克隆性定植。来自孕妇的分离株与近10年前获得的UTI PFGE聚集型A和E有100%的相似性，表明腐生链霉菌在时间上是持续的。海滩分离株的耐药性反映了人类分离株的特征。综上所述，结果表明人类和环境隔离的来源是共享的。
Staphylococcus saprophyticus is an important agent of urinary tract infection (UTI) in young women, but information about this pathogen in human microbiota and in common environment is lacking. The aim of this study was to characterize S. saprophyticus isolates from genitoanal microbiota of 621 pregnant women, 10 minas cheese packs, and five beaches in Rio de Janeiro city and compare PFGE profiles of these isolates with five UTI PFGE clusters described in this city. We investigated 65 S. saprophyticus isolates from microbiota, 13 from minas cheese, and 30 from beaches and 32 UTI isolates. Antimicrobial resistance was determined by disk diffusion, MIC by agar dilution, and PCR. Erythromycin-resistance genes erm(C), msr(A), msr(B), mph(C), and lin(A) were found in 93% of isolates. Trimethoprim-sulfamethoxazole resistance correlated with dfrG or dfrA genes. Three cefoxitin-resistant isolates carried the mecA gene. All isolates obtained from cheese were susceptible to all antimicrobial agents. Six of 10 pregnant women with >1 isolate had monoclonal colonization. Isolates from pregnant women shared 100% similarity with UTI PFGE cluster types A and E obtained almost 10 years previously, suggesting temporal persistence of S. saprophyticus. Antimicrobial resistance of beach isolates reflected the profiles of human isolates. Taken together, results indicate a shared source for human and environmental isolates.