Cystatin C in cerebrospinal fluid and multiple sclerosis
Cystatin C in cerebrospinal fluid and multiple sclerosis
复制标题
DOI：
10.1002/ana.20945
复制
发表时间：
2007-08-01
影响因子：
11.2
通讯作者：
Blennow, Kaj
中科院分区：
文献类型：
--
作者：
Hansson, Sara F.;Simonsen, Anja Hviid;Blennow, Kaj
目的：最近一项使用表面增强激光解吸/电离飞行时间分析脑脊液的研究确定了12.5kDa截短的半胱氨酸蛋白酶抑制剂C14（CysC）亚型作为多发性硬化症（MS）的特异性生物标志物。方法：表面增强激光解吸/电离飞行时间分析来自43名MS患者和46名健康对照受试者的脑脊液样本。结果：全长CysC（13.4kDa）浓度在MS和对照样品中相似。12.5kDa CysC蛋白是在-20 ℃下储存期间通过N-末端切割由全长CysC产生的。解释：12.5kDa CysC同种型是储存相关的人工产物，不能用作MS的诊断标志物。
Objective: A recent study using surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid identified a 12.5kDa truncated isoform of cystatin C 14 (CysC) as a specific biomarker for multiple sclerosis (MS).Methods: Surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid samples from 43 MS patients and 46 healthy control subjects.Results: Full-length CysC (13.4kDa) concentration was similar in MS and control samples. The 12.5kDa CysC protein was produced from full-length CysC by N-terminal cleavage during storage at -20 degrees C.Interpretation: The 12.5kDa CysC isoform is a storage-related artifact and is not useful as a diagnostic marker for MS.