Entropic trapping and escape of long DNA molecules at submicron size constriction
Entropic trapping and escape of long DNA molecules at submicron size constriction
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DOI：
10.1103/physrevlett.83.1688
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发表时间：
1999-08-23
影响因子：
8.6
通讯作者：
Craighead, HG
中科院分区：
文献类型：
--
作者：
Han, J;Turner, SW;Craighead, HG
我们研究了 DNA 分子在电场驱动下通过具有 90 nm 尺寸收缩的微加工通道的过程。 DNA分子被熵捕获在收缩处并以特征寿命逃逸。与直觉相反的是，较长的 DNA 比较短的 DNA 逃离熵陷阱的速度更快。 DNA分子通过将其单体拉伸到收缩处来克服熵屏障，这导致DNA逃逸的能量屏障与链长度无关。
We studied the passage of DNA molecules, driven by an electric field, through a microfabricated channel with 90 nm size constrictions. DNA molecules were entropically trapped at the constriction and escaped with a characteristic lifetime. Counterintuitively, longer DNA were found to escape entropic traps faster than shorter ones. DNA molecules overcome the entropic barrier by stretching their monomers into the constriction, which results in the fact that the energy barrier for DNA escape is independent of the chain length.