Vaccinia virus replication is not affected by APOBEC3 family members
Vaccinia virus replication is not affected by APOBEC3 family members
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DOI：
10.1186/1743-422x-3-86
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发表时间：
2006-10-19
期刊：
影响因子：
4.8
通讯作者：
Schnierle, Barbara S.
中科院分区：
文献类型：
--
作者：
Kremer, Melanie;Suezer, Yasemin;Schnierle, Barbara S.
背景：APOBEC3G 蛋白代表了一种针对逆转录病毒感染的新型先天防御机制。它促进逆转录病毒感染早期步骤中单链 cDNA 中间体中胞嘧啶残基的脱氨基作用。大多数痘病毒基因组相对富含 A/T，这可能表明 APOBEC3G 诱导的突变压力。此外，痘病毒仅在 APOBEC3G 所在的细胞质中复制。因此，分析痘苗病毒复制是否受到 APOBEC3G 的影响是很诱人的。结果：然而，痘苗病毒（一种原型痘病毒）的复制在表达 APOBEC3G 的细胞中并未受到抑制，APOBEC3 家族的其他成员也不会改变痘苗病毒的复制。 HIV 通过诱导 APOBEC3G 降解来抵消 APOBEC3G。然而，Western blot分析显示APOBEC3G蛋白水平不受痘苗病毒感染的影响。结论：数据表明APOBEC3G不是痘苗病毒复制的限制因素，痘苗病毒也不能降解APOBEC3G。
Background: The APOBEC3G protein represents a novel innate defense mechanism against retroviral infection. It facilitates the deamination of the cytosine residues in the single stranded cDNA intermediate during early steps of retroviral infection. Most poxvirus genomes are relatively A/T-rich, which may indicate APOBEC3G-induced mutational pressure. In addition, poxviruses replicate exclusively in the cytoplasm where APOBEC3G is located. It was therefore tempting to analyze whether vaccinia virus replication is affected by APOBEC3G.Results: The replication of vaccinia virus, a prototype poxvirus, was not, however, inhibited in APOBEC3G-expressing cells, nor did other members of the APOBEC3 family alter vaccinia virus replication. HIV counteracts APOBEC3G by inducing its degradation. However, Western blot analysis showed that the levels of APOBEC3G protein were not affected by vaccinia virus infection.Conclusion: The data indicate that APOBEC3G is not a restriction factor for vaccinia virus replication nor is vaccinia virus able to degrade APOBEC3G.