Organization of geniculate and trigeminal ganglion cells innervating single fungiform taste papillae: a study with tetramethylrhodamine dextran amine labeling.
Organization of geniculate and trigeminal ganglion cells innervating single fungiform taste papillae: a study with tetramethylrhodamine dextran amine labeling.
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支配单个菌状味觉乳头的膝状神经节和三叉神经节细胞的组织：四甲基罗丹明葡聚糖胺标记的研究。
DOI：
10.1016/s0306-4522(99)00115-3
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发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Yao,B
中科院分区：
文献类型：
--
作者：
Whitehead,MC;Ganchrow,JR;Ganchrow,D;Yao,B
单个味觉神经纤维分支并支配几个味蕾。反过来，个别味蕾可以接受来自众多味觉神经纤维的神经支配。为了评估菌状乳头味蕾的感觉神经支配的模式，我们使用离子电渗荧光注射逆行标记的纤维，支配仓鼠的单一味觉乳头。对于每只动物，通过胚芽孔向单个味觉乳头注射3.3%四甲基罗丹明葡聚糖胺。在舌尖（距舌尖0.5-1.5mm）或更靠后（距舌尖1.5-3.0mm）处注射菌状乳头。存活1 - 7天后，分别对膝状体和三叉神经节以及舌进行切片并检查标记细胞和纤维。对支配单个味觉乳头的膝状体细胞的数量和分布的分析表明：（1）15±4（S.D.）神经节细胞会聚以神经支配单个菌状味蕾;（ii）神经节细胞神经支配前部芽（范围：13-35个细胞）多于后部芽（范围：5 - 12个细胞），这部分地可能与芽体积（μm3）有关;以及（iii）神经节胞体神经支配单个味蕾广泛地分散在膝状神经节内。对舌中标记纤维的分析表明，位于注射味蕾2 mm内的2至8个味蕾与注射味蕾共享侧支神经支配。由于所有带有标记纤维的芽都位于非常接近的位置（在2 mm半径内），因此广泛分散的膝状神经节细胞会聚以支配紧密间隔的菌状味蕾。三叉神经节（下颌分裂）细胞也标记在每一种情况下，与膝状神经节，分散的细胞体的位置和侧支乳头之间的模式进行了观察。这项研究表明，四甲基罗丹明葡聚糖胺，选择性地应用于个人外周受体终末器官的离子电渗，有效地定位在两个不同的神经节，这些网站的项目感觉神经节细胞。此外，标记物显示与标记的纤维相关的感觉传入的侧支和由这些侧支支配的附近的受体区域。单个或集群的受体细胞，以及确定的感觉传入的标记，提供了未来的可能性，结合这种技术与免疫细胞化学，以建立神经支配模式与神经递质和亲神经性物质的关系内确定的细胞。
Single gustatory nerve fibers branch and innervate several taste buds. In turn, individual taste buds may receive innervation from numerous gustatory nerve fibers. To evaluate the pattern of sensory innervation of fungiform papilla-bearing taste buds, we used iontophoretic fluorescent injection to retrogradely label the fibers that innervate single taste papillae in the hamster. For each animal, a single taste papilla was injected through the gemmal pore with 3.3% tetramethylrhodamine dextran amine. Fungiform papillae either at the tongue tip (0.5–1.5mm from the tip) or more posteriorly (1.5–3.0mm from the tip) were injected. After one to seven days survival, the geniculate and trigeminal ganglia and the tongue were sectioned and examined for labeled cells and fibers, respectively. Analysis of the number and topographic distribution of geniculate cells innervating single taste papillae, shows that: (i) 15±4 (S.D.) ganglion cells converge to innervate a single fungiform taste bud; (ii) more ganglion cells innervate anterior- (range: 13–35 cells) than posterior-lying buds (range: five to 12 cells), which, in part, may be related to bud volume (μm3); and (iii) ganglion somata innervating a single taste bud are scattered widely within the geniculate ganglion. Analysis of labeled fibers in the tongue demonstrated that two to eight taste buds located within 2mm of the injected taste bud share collateral innervation with the injected taste bud. Since all buds with labeled fibers were located in close proximity (within a 2-mm radius), widely dispersed geniculate ganglion cells converge to innervate closely spaced fungiform taste buds. Trigeminal ganglion (mandibular division) cells were also labeled in every case and, as with the geniculate ganglion, a dispersed cell body location and collateralization pattern among papillae were observed. This study shows that iontophoresis of tetramethylrhodamine dextran amine, selectively applied to individual peripheral receptor end-organs, effectively locates sensory ganglion cells in two different ganglia that project to these sites. Moreover, the marker demonstrates collateral branches of sensory afferents associated with the labeled fibers and the nearby receptor areas innervated by these collaterals. The labeling of single or clusters of receptor cells, as well as identified sensory afferents, affords future possibilities for combining this technique with immunocytochemistry to establish the relationships of innervation patterns with neurotransmitters and neurotropic substances within identified cells.