Changes in the basement membrane zone components during skeletal muscle fiber degeneration and regeneration.
Changes in the basement membrane zone components during skeletal muscle fiber degeneration and regeneration.
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DOI：
10.1083/jcb.97.4.957
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发表时间：
1983-10
影响因子：
7.8
通讯作者：
Zalewski, A A
中科院分区：
文献类型：
--
作者：
Gulati, A K;Reddi, A H;Zalewski, A A
骨骼肌纤维的基底膜被认为在肌纤维变性过程中保持不变，并作为管状结构，在其中发生新肌纤维的再生。在本研究中，我们描述了大分子的变化，在肌肉变性和再生过程中的基底膜区，使用特异性抗体对IV型和V型胶原蛋白，层粘连蛋白，硫酸乙酰肝素蛋白聚糖和刀豆球蛋白A（Con A）的结合免疫荧光监测。采用大鼠趾长伸肌自体移植诱导骨骼肌再生。在此过程之后，肌纤维退化;这之后是肌卫星细胞活化、增殖和融合，导致形成成熟为肌纤维的新肌管。在正常肌肉中，IV型和V型胶原，层粘连蛋白，硫酸乙酰肝素蛋白多糖，和Con A结合的分布被认为是在细胞周围的基底膜区域。在自体移植的肌肉中，基底膜区的各种成分消失，留下一些仍然结合Con A的无法识别的成分。再生肌管周围重新出现一个新的基底膜（区），在再生肌肉的成熟过程中持续存在。再生肌纤维中各种基底膜成分的分布与正常肌相似。根据我们目前和以前的研究（Gulati，A.K.，A.H. Reddi和AA Zalewski，1982，Anat.Rec.204：175-183），看来一些原始基底膜区组分在肌纤维变性和初始再生期间消失。随着新的基底膜的形成，其成分重新出现并持续存在于成熟的肌纤维中。我们的结论是，骨骼肌纤维基底膜（区）是不是一个静态的结构，如以前认为的，而是它的成分变化相当迅速，在肌纤维变性和再生。
The basement membrane of skeletal muscle fibers is believed to persist unchanged during myofiber degeneration and act as a tubular structure within which the regeneration of new myofibers occurs. In the present study we describe macromolecular changes in the basement membrane zone during muscle degeneration and regeneration, as monitored by immunofluorescence using specific antibodies against types IV and V collagen, laminin, and heparan sulfate proteoglycan and by the binding of concanavalin A (Con A). Skeletal muscle regeneration was induced by autotransplantation of the extensor digitorum longus muscle in rats. After this procedure, the myofibers degenerate; this is followed by myosatellite cell activation, proliferation, and fusion, resulting in the formation of new myotubes that mature into myofibers. In normal muscle, the distribution of types IV and V collagen, laminin, heparan sulfate proteoglycan, and Con A binding was seen in the pericellular basement membrane region. In autotransplanted muscle, the various components of the basement membrane zone disappeared, leaving behind some unidentifiable component that still bound Con A. Around the regenerated myotubes a new basement membrane (zone) reappeared, which persisted during maturation of the regenerating muscle. The distribution of various basement membrane components in the regenerated myofibers was similar to that seen in the normal muscle. Based on our present and previous study (Gulati, A.K., A.H. Reddi, and A.A. Zalewski, 1982, Anat. Rec. 204:175-183), it appears that some of the original basement membrane zone components disappear during myofiber degeneration and initial regeneration. As a new basement membrane develops, its components reappear and persist in the mature myofibers. We conclude that skeletal muscle fiber basement membrane (zone) is not a static structure as previously thought, but rather that its components change quite rapidly during myofiber degeneration and regeneration.