Sex-differential modulation of visceral pain by brain derived neurotrophic factor (BDNF) in rats
Sex-differential modulation of visceral pain by brain derived neurotrophic factor (BDNF) in rats
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DOI：
10.1016/j.neulet.2010.05.013
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发表时间：
2010-07-12
影响因子：
2.5
通讯作者：
Dai, Ru-Ping
中科院分区：
文献类型：
--
作者：
Li, Fang;Zhang, Jian-Wei;Dai, Ru-Ping
尽管脑源性神经营养因子（BDNF）已被报道与内脏疼痛的发生有关，但BDNF在疼痛中的作用是否与性别有关仍有待确定。本研究探讨了BDNF对不同性别大鼠内脏疼痛的影响。通过腹腔注射乙酸（AA）建立了sd - dawley大鼠内脏痛模型：雄性、雌性和卵巢切除（OVX）的雌性。通过统计腹腔注射AA后60min内腹部收缩次数来评估疼痛行为指数。在AA注射前1小时给予抗BDNF抗体（BDNF），以检测BDNF在内脏疼痛中的作用。注射AA后，所有大鼠的腹部收缩次数均显著增加，但雌性表现出比雄性更严重的疼痛行为。对aa诱导的恶性反应的高敏感性被OVX减弱。抗bdnf抗体预处理显著增强了雄性小鼠的恶性反应，而雌性小鼠的恶性反应减弱。虽然外源性BDNF给药没有改变AA注射诱导的雌性有害反应，但BDNF预处理在雄性OVX中减弱了有害反应，但在雌性OVX中加剧了有害反应。本研究提示AA注射引起的内脏疼痛存在性别差异。此外，BDNF对内脏疼痛的调节也是性别依赖的，即BDNF在雌性大鼠中促进内脏疼痛，而在雄性大鼠中表现出相反的作用。我们的结果可能对临床疼痛的管理有重要的意义。2010爱思唯尔爱尔兰有限公司版权所有。
In spite of the fact that brain derived neurotrophic factor (BDNF) has been reported to be implicated in the development of visceral pain, it remains to be determined whether the role of BDNF in pain is gender dependent. The present study investigated the effect of BDNF on visceral pain in different gender rats. A model for visceral pain was established by intraperitoneal (i.p.) injection of acetic acid (AA) into Sprague-Dawley rats: males, females and females with an ovariectomy (OVX). The pain behavior index was assessed by counting the number of abdominal contractions for 60 min after i.p. injection of AA. Anti-BDNF antibody, or BDNF, was administered 1 h before the AA injection to examine the role of BDNF in visceral pain. After the AA injection, the number of abdominal contraction was dramatically increased in all rats but females showed more severe pain behavior than males. The higher sensitivity to AA-induced nocifensive response was attenuated by OVX. Pretreatment with anti-BDNF antibody significantly exacerbated the nocifensive response in males but attenuated it in females. While exogenous BDNF administration did not alter AA injection-induced nocifensive response in females, BDNF pretreatment attenuated the nocifensive response in males but exacerbated it in females with OVX. The present study suggests there is a gender dichotomy in visceral pain induced by AA injection. In addition, the modulation of visceral pain by BDNF is also sex dependent, i.e., BDNF facilitates the visceral pain in female rats but displays an opposite effect in male rats. Our results may have important implications in the management of clinical pain. (c) 2010 Elsevier Ireland Ltd. All rights reserved.