A and D genomes spatial separation at somatic metaphase in tetraploid cotton: evidence for genomic disposition in a polyploid plant
A and D genomes spatial separation at somatic metaphase in tetraploid cotton: evidence for genomic disposition in a polyploid plant
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四倍体棉花体细胞中期的 A 和 D 基因组空间分离：多倍体植物基因组分布的证据。
DOI：
10.1111/tpj.13074
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发表时间：
2015-12-01
期刊：
影响因子：
7.2
通讯作者：
Wang, Kai
中科院分区：
文献类型：
--
作者：
Han, Jinlei;Zhou, Baoliang;Wang, Kai
在四倍体棉花（AADD）中期平板上分析了染色体配置。在中期，A和D两个亚基因组呈放射状分离，小的D亚基因组染色体倾向于集中在中心，大的A亚基因组染色体分散在外围。虽然人工六倍体（AADDGG）的有序染色体排列受到干扰，但通过与四倍体棉花（AADD）回交，去除大部分附加基因组（GG）染色体后，分离模式可以恢复。在合成四倍体棉花（AAGG）中也发现了类似的基因组分离现象。这些结果表明，该基因组分离模式可以在四倍体棉花形成后立即建立，并在四倍体棉花中稳定遗传。鉴于其他植物和动物亲本基因组分离的证据，我们推测基因组分离可能是二倍体和多倍体物种的正常现象。这些发现将有助于揭示植物细胞中的染色体构象。
Chromosomal dispositions were analyzed on the metaphase plate of tetraploid cotton (AADD). At metaphase, the two subgenomes, A and D, were separated in a radial pattern in which the small D subgenome chromosomes tended to concentrate at the center and the large A subgenome chromosomes were scattered about the periphery on the metaphase plate. Although the ordered chromosome arrangement was disturbed in an artificial hexaploid (AADDGG), the separation pattern could be recovered after the majority of the additional genome (GG) chromosomes were removed by backcrossing the artificial hexaploid with the tetraploid cotton (AADD). A similar genome separation phenomenon was also found in synthesized tetraploid cotton (AAGG). These results indicate that the genome separation pattern could be established immediately after tetraploid cotton formation and could be stably inherited in tetraploid cotton. Given the evidence of parental genome separation in other plants and animals, we speculated that genome separation might be a normal phenomenon in diploid and polyploid species. These finding will shed light on the chromosome conformation in plant cells.