Use of nitroxides for assessing perfusion, oxygenation, and viability of tissues: In vivo EPR and MRI studies
Use of nitroxides for assessing perfusion, oxygenation, and viability of tissues: In vivo EPR and MRI studies
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DOI：
10.1002/mrm.1910350113
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发表时间：
1996-01-01
影响因子：
3.3
通讯作者：
Swartz, HM
中科院分区：
文献类型：
--
作者：
Gallez, B;Bacic, G;Swartz, HM
在小鼠体内组织中，通过跟踪直接注射到组织中的氮氧自由基的EPR谱的强度和形状的变化，使用低频技术，同时测量相对灌注、pO（2）和生物还原。（1.1GHz）局域EPR光谱，用正常和血流受限的腓肠肌，表明组织中氮氧化物的EPR信号的减少主要是由于再到灌注，重新分配氮氧自由基pO（2）的变化通过线宽的变化来反映;只有具有窄线的全氘代氮氧自由基才是该参数的适当指示剂。将该技术应用于实验小鼠肿瘤（MTG-B和RIF-1）以确定这些相对低氧模型肿瘤系统中的灌注和pO（2）。利用氮氧化物的顺磁特性来增强T-1加权MR图像，证明了个体肿瘤灌注的异质性。
Relative perfusion, pO(2), and bioreduction were measured simultaneously in vivo in tissues in mice by following changes in the intensity and shape of the EPR spectra of nitroxides injected directly into the tissues, using low frequency (1.1 GHz) localized EPR spectroscopy, Using normal and blood flow restricted gastrocnemius muscles it was shown that the decrease of the EPR signals of the nitroxides in tissues was due principally to perfusion, which redistributed the nitroxides. Changes in pO(2) were reflected by changes of the linewidth; only a perdeuterated nitroxide with a narrow line was an adequate indicator for this parameter, This technique was applied experimental murine tumors (MTG-B and RIF-1) to determine the perfusion and pO(2) in these relatively hypoxic model tumor systems. Using the paramagnetic properties of the nitroxides to enhance T-1-weighted MR images, heterogeneity in perfusion in individual tumors was demonstrated.