Quantification of differential ErbB1 and ErbB2 cell surface expression and spatial nanoclustering through plasmon coupling.
Quantification of differential ErbB1 and ErbB2 cell surface expression and spatial nanoclustering through plasmon coupling.
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DOI：
10.1021/nl3012227
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发表时间：
2012-06-13
期刊：
影响因子：
10.8
通讯作者：
Reinhard BM
中科院分区：
文献类型：
--
作者：
Wang J;Yu X;Boriskina SV;Reinhard BM
细胞表面受体在细胞信号传导和通讯中发挥着普遍的作用，其表达水平是许多疾病的重要生物标志物。然而，表达水平只是决定受体生理活性的一个因素。对于一些表面受体，它们在细胞表面上的分布，特别是它们的聚集，提供了额外的调节机制。为了获得这种空间信息，需要稳健的测定，其提供对纳米长度尺度上的细胞表面受体的组织的详细了解。在这篇手稿中，我们证明通过结合散射光谱，电子显微镜，和推广的多粒子米氏理论（GMT）模拟表明，Au纳米粒子（NP）的密度和形貌依赖的光谱响应与表皮生长因子受体ErbB1和ErbB2结合的免疫标记编码两种受体在不同的非配体细胞中的细胞表面表达和空间聚集的定量信息。体外癌细胞系（SKBR 3、MCF 7、A431）。针对ErbB1和ErbB2的NP在不同NP浓度下的集体光谱响应的系统表征表明ErbB1和ErbB2在过表达这些受体的细胞系中的大规模组织的差异。扫描电子显微镜（SEM）中的验证实验证实，在总体相当的NP表面密度下，靶向A431上ErbB1的NP比结合SKBR 3或MCF7上ErbB2的NP更强烈地聚集。这一发现是一致的存在较大的受体簇ErbB1比ErbB2在各自的细胞的质膜。
Cell surface receptors play ubiquitous roles in cell signaling and communication and their expression levels are important biomarkers for many diseases. Expression levels are, however, only one factor that determines the physiological activity of a receptor. For some surface receptors their distribution on the cell surface, especially their clustering, provides additional mechanisms for regulation. To access this spatial information robust assays are required that provide detailed insight into the organization of cell surface receptors on nanometer length scales. In this manuscript we demonstrate through combination of scattering spectroscopy, electron microscopy, and generalized multiple particle Mie theory (GMT) simulations that the density- and morphology-dependent spectral response of Au nanoparticle (NP) immunolabels bound to the epidermal growth factor receptors ErbB1 and ErbB2 encodes quantitative information of both the cell surface expression and spatial clustering of the two receptors in different unliganded in vitro cancer cell lines (SKBR3, MCF7, A431). A systematic characterization of the collective spectral responses of NPs targeted at ErbB1 and ErbB2 at various NP concentrations indicates differences in the large-scale organization of ErbB1 and ErbB2 in cell lines that overexpress these receptors. Validation experiments in the scanning electron microscope (SEM) confirm that NPs targeted at ErbB1 on A431 are more strongly clustered than NPs bound to ErbB2 on SKBR3 or MCF7 at overall comparable NP surface densities. This finding is consistent with the existence of larger receptor clusters for ErbB1 than for ErbB2 in the plasma membranes of the respective cells.
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影响因子：
17.1
作者：
Kennedy, David C.;Tay, Li-Lin;Pezacki, John Paul
通讯作者：
Pezacki, John Paul
DOI：
10.1021/la200659z
发表时间：
2011-06-21
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Ma LL;Tam JO;Willsey BW;Rigdon D;Ramesh R;Sokolov K;Johnston KP
通讯作者：
Johnston KP
影响因子：
56.9
作者：
Prodan, E;Radloff, C;Nordlander, P
通讯作者：
Nordlander, P
影响因子：
10.8
作者：
Jain, Prashant K.;Huang, Wenyu;El-Sayed, Mostafa A.
通讯作者：
El-Sayed, Mostafa A.
影响因子：
3.5
作者：
Mayawala, K;Vlachos, DG;Edwards, JS
通讯作者：
Edwards, JS