Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.
Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.
复制标题
猿猴病毒 40 早期 mRNA 推定启动子区域的缺失不会消除 T 抗原表达。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
P. Chambon
中科院分区：
文献类型：
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作者：
C. Benoist;P. Chambon
将猴病毒40（SV40）的早期基因插入pBR 322中，构建重组质粒。当它被导入真核细胞时，SV40早期基因得到表达。我们已经制造了该质粒的缺失突变体，其中SV 40早期mRNA的主要帽位点连同上游的一些序列一起被沿着去除。缺失的序列似乎与早期基因表达有关，但这并不一定意味着它们在野生型SV 40的转录起始中没有功能。
A recombinant plasmid was constructed by insertion of the early genes of simian virus 40 (SV40) into pBR322. When it was introduced into eukaryotic cells, the SV40 early genes were expressed. We have made deletion mutants of this plasmid, from which the major cap sites of SV40 early mRNAs have been removed along with some of the sequences upstream. The deleted sequences appear to be dispensable for early gene expression, but this does not necessarily imply that they serve no function in the initiation of transcription on wild-type SV40.