Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASES1 and 2 are essential for tapetum development and microspore maturation
Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASES1 and 2 are essential for tapetum development and microspore maturation
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DOI：
10.1105/tpc.105.036731
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发表时间：
2005-12-01
期刊：
影响因子：
11.6
通讯作者：
Schroeder, JI
中科院分区：
文献类型：
--
作者：
Colcombet, J;Boisson-Dernier, A;Schroeder, JI
在拟南芥富含亮氨酸的重复序列受体激酶家族的>200个成员中，只有少数已被功能性地表征。在这里，我们报告了体细胞胚胎发生受体激酶1（SERK1）和SERK2基因在花药发育中的关键功能。SERK1和SERK2在第6期花药中广泛表达，以后在绒毡层细胞中表达更集中。而serk1和serk2单插入突变体没有表现出发育表型，serk1 serk2双突变体不能产生种子，因为缺乏花粉发育的突变花药。在幼芽中，双突变体花药发育正常，但serk1 serk2小孢子囊产生更多的造孢细胞，无法发展超出减数分裂。此外，serk1 serk2双突变体只有三个细胞层周围的造孢细胞团，而野生型花药开发四个细胞层。进一步的共聚焦显微镜和分子生物学分析表明，serk1 serk2双突变体花药绒毡层细胞层发育不全，是导致小孢子败育和雄性不育的主要原因。综上所述，这些发现表明，SERK1和SERK2受体激酶冗余功能作为一个重要的控制点孢子体发育控制雄配子体生产。
Among the >200 members of the leucine-rich repeat receptor kinase family in Arabidopsis thaliana, only a few have been functionally characterized. Here, we report a critical function in anther development for the SOMATIC EMBRYOGENESIS RECEPTOR KINASE1 (SERK1) and SERK2 genes. Both SERK1 and SERK2 are expressed widely in locules until stage 6 anthers and are more concentrated in the tapetal cell layer later. Whereas serk1 and serk2 single insertion mutants did not show developmental phenotypes, serk1 serk2 double mutants were not able to produce seeds because of a lack of pollen development in mutant anthers. In young buds, double mutant anthers developed normally, but serk1 serk2 microsporangia produced more sporogenous cells that were unable to develop beyond meiosis. Furthermore, serk1 serk2 double mutants developed only three cell layers surrounding the sporogenous cell mass, whereas wild-type anthers developed four cell layers. Further confocal microscopic and molecular analyses showed that serk1 serk2 double mutant anthers lack development of the tapetal cell layer, which accounts for the microspore abortion and male sterility. Taken together, these findings demonstrate that the SERK1 and SERK2 receptor kinases function redundantly as an important control point for sporophytic development controlling male gametophyte production.