Mammalian neo-oogenesis and expression of meiosis-specific protein SCP3 in adult human and monkey ovaries
Mammalian neo-oogenesis and expression of meiosis-specific protein SCP3 in adult human and monkey ovaries
复制标题
DOI：
10.4161/cc.7.5.5453
复制
发表时间：
2008-03-01
期刊：
影响因子：
4.3
通讯作者：
Dominguez, Roberto
中科院分区：
文献类型：
--
作者：
Bukovsky, Antonin;Caudle, Michael R.;Dominguez, Roberto
包括人类在内的哺乳动物雌性成年期间的新卵子发生和卵泡更新的概念是一个新概念，对于卵巢生理学和哺乳动物生殖生物学具有重要意义。我们实验室之前的观察表明，白膜中的间充质细胞是成人卵巢中颗粒细胞和生殖细胞的双能祖细胞。在本研究中，我们证明抗减数分裂进入联会复合蛋白 3 (SCP3)（减数分裂标记物）的抗体与白膜和卵巢表面上皮片段以及功能性人类和猴卵巢中一些原始卵泡的卵母细胞表现出反应性。这些观察结果表明 SCP3 在成年人和猴子的卵巢中表达。减数分裂活动的准备可能已经在白膜干细胞水平上发生，并且减数分裂前期活动可能在新形成的原始卵泡的卵母细胞中继续并终止。
The concept of neo-oogenesis and follicular renewal during adulthood in mammalian females, including humans, is a novel concept with major significance for ovarian physiology and mammalian reproductive biology. Previous observations from our laboratory demonstrated that mesenchymal cells in the tunica albuginea are bipotent progenitors for both granulosa and germ cells in adult human ovaries. In the present studies, we demonstrate that the antibodies against meiotic entry synaptonemal complex protein 3 (SCP3)-a marker for meiosis, showed reactivity with segments of tunica albuginea and ovarian surface epithelium, and in oocytes of some primordial follicles in functional human and monkey ovaries. These observations suggest that SCP3 is expressed in adult human and monkey ovaries. Preparation for meiotic activity may have already occurred at the level of tunica albuginea stem cells, and meiotic prophase activity may continue and terminate in oocytes of newly formed primordial follicles.