Induced pluripotent stem cell lines derived from human somatic cells
Induced pluripotent stem cell lines derived from human somatic cells
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DOI：
10.1126/science.1151526
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发表时间：
2007-12-21
期刊：
影响因子：
56.9
通讯作者：
Thomson, James A.
中科院分区：
文献类型：
--
作者：
Yu, Junying;Vodyanik, Maxim A.;Thomson, James A.
体细胞核移植可使哺乳动物卵母细胞中存在的反式作用因子将体细胞核重编程至未分化状态。我们发现四种因子（OCT4、SOX2、NANOG和LIN28）足以将人类体细胞重编程为多能干细胞，这些多能干细胞展现出胚胎干细胞（ES）的基本特征。这些诱导性多能人类干细胞具有正常的核型，表达端粒酶活性，表达细胞表面标志物以及表征人类ES细胞的基因，并保持分化为所有三个主要胚层的晚期衍生物的发育潜能。一旦技术限制（例如，通过病毒整合产生的突变）被消除，此类诱导性多能人类细胞系应可用于构建新的疾病模型、药物研发以及移植医学等领域。
Somatic cell nuclear transfer allows trans- acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors ( OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem ( ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations ( for example, mutation through viral integration) are eliminated.