Ferulic Acid Suppresses Amyloid β Production in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide.
Ferulic Acid Suppresses Amyloid β Production in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide.
复制标题
DOI：
10.1155/2017/5343010
复制
发表时间：
2017
影响因子：
--
通讯作者：
Fujii N
中科院分区：
文献类型：
--
作者：
Nagai N;Kotani S;Mano Y;Ueno A;Ito Y;Kitaba T;Takata T;Fujii N
众所周知，氧化应激诱导脑、透镜和视网膜中淀粉样蛋白β（Aβ）的产生，导致年龄相关疾病。在本研究中，我们研究了阿魏酸对H2 O2刺激的人透镜上皮（HLE）SRA 01/04细胞中Aβ水平的影响。通过ELISA测定三种类型的Aβ肽（Aβ1-40、Aβ1-42和Aβ1-43），并通过定量实时RT-PCR测定与Aβ产生（APP、BACE 1和PS蛋白）和降解（ADAM 10、NEP和ECE 1蛋白）相关的表达蛋白的mRNA水平。H2 O2刺激可增加与Aβ生成相关蛋白的基因表达，导致3种Aβ肽的生成。0.1 μM阿魏酸可抑制H2 O2刺激的HLE细胞中与三种Aβ肽分泌相关的基因表达和蛋白质产量的增加。这些结果为阿魏酸对透镜上皮细胞的抗氧化作用提供了证据。
It is well known that oxidative stresses induce the production of amyloid β (Aβ) in the brain, lens, and retina, leading to age-related diseases. In the present study, we investigated the effects of ferulic acid on the Aβ levels in H2O2-stimulated human lens epithelial (HLE) SRA 01/04 cells. Three types of Aβ peptides (Aβ1-40, Aβ1-42, and Aβ1-43) were measured by ELISA, and the levels of mRNA for the expressed proteins related to Aβ production (APP, BACE1, and PS proteins) and degradation (ADAM10, NEP, and ECE1 proteins) were determined by quantitative real-time RT-PCR. H2O2 stimulation augmented gene expression of the proteins related to Aβ production, resulting in the production of three types of Aβ peptides. Treatment with 0.1 μM ferulic acid attenuated the augmentations of gene expression and production of the proteins related to the secretion of three types of Aβ peptides in the H2O2-stimulated HLE cells. These results provided evidence of antioxidative functions of ferulic acid for lens epithelial cells.